Troponin C-I Human
Greater than 90.0% as determined by Analysis by SDS-PAGE.
Description
Recombinant Human Cardiac Troponin C-I complex produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of approximately 50kDa. The Molar ratio on cTnC to cTnI is 1:1.
The Cardiac Troponin C-I is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The cardiac troponin complex is a critical component of the contractile apparatus in cardiac muscle cells. It plays a pivotal role in the regulation of muscle contraction in response to calcium ions. The complex is composed of three subunits: troponin C (cTnC), troponin I (cTnI), and troponin T (cTnT). Each subunit has a unique function that contributes to the overall mechanism of muscle contraction.
- Troponin C (cTnC): This subunit binds calcium ions, which is essential for the initiation of muscle contraction. The binding of calcium to cTnC induces a conformational change that allows the interaction of the other troponin subunits with actin and myosin, the primary proteins involved in muscle contraction.
- Troponin I (cTnI): This subunit inhibits the interaction between actin and myosin, preventing muscle contraction in the absence of calcium. When calcium binds to cTnC, the inhibitory effect of cTnI is relieved, allowing muscle contraction to proceed.
- Troponin T (cTnT): This subunit binds the troponin complex to tropomyosin, a protein that runs along the length of the actin filament. Tropomyosin blocks the binding sites for myosin on the actin filament, and its position is regulated by the troponin complex.
The recombinant human cardiac troponin C-I complex is a synthetic version of the naturally occurring complex, produced using recombinant DNA technology. This technology involves inserting the genes encoding cTnC and cTnI into a host organism, such as bacteria or yeast, which then produces the proteins. These proteins are subsequently purified and combined to form the recombinant complex.
The recombinant cardiac troponin C-I complex is used in various research and clinical applications:
- Diagnostic Tools: Cardiac troponins, particularly cTnI and cTnT, are widely used as biomarkers for the diagnosis of acute myocardial infarction (heart attack) and other forms of cardiac injury. Elevated levels of these proteins in the blood indicate damage to the heart muscle.
- Research: The recombinant complex is used in studies to understand the molecular mechanisms of muscle contraction and the regulation of cardiac function. It is also used to investigate the effects of mutations and post-translational modifications on the function of the troponin complex.
- Therapeutic Development: Understanding the structure and function of the troponin complex can aid in the development of new therapies for heart disease. For example, drugs that modulate the activity of the troponin complex could potentially be used to treat conditions such as heart failure.
