Troponin C-I-T Human
Greater than 90.0% as determined by SDS-PAGE.
Description
Recombinant Human Cardiac Troponin C-I-T complex produced in E.Coli is a single, non-glycosylated, polypeptide chain having the Molar ratio on cTnC to cTnI to cTnT of 1:1:1. Mw is approximately 90kDa.
The Cardiac Troponin C-I-T complex is purified by proprietary chromatographic techniques
Product Specs
Product Science Overview
The cardiac troponin complex is a crucial component in the regulation of cardiac muscle contraction. It is composed of three subunits: troponin C, troponin I, and troponin T. Each subunit plays a unique role in the function of the complex, which is essential for the proper functioning of the heart muscle.
- Troponin C: This subunit binds calcium ions, which is a critical step in the initiation of muscle contraction. The binding of calcium to troponin C induces a conformational change that allows the interaction of the other subunits with actin and myosin, the proteins responsible for muscle contraction.
- Troponin I: This subunit inhibits the interaction between actin and myosin, preventing muscle contraction in the absence of calcium. It acts as an inhibitory protein that ensures muscle relaxation when calcium levels are low.
- Troponin T: This subunit binds the troponin complex to tropomyosin, a protein that runs along the length of the actin filament. Troponin T anchors the complex in place, allowing it to effectively regulate muscle contraction.
The cardiac troponin complex is a well-established biomarker for the diagnosis of acute myocardial infarction (heart attack). When the heart muscle is damaged, troponin is released into the bloodstream, and its levels can be measured to assess the extent of the damage. Among the three subunits, troponin I is considered the most specific and reliable marker for cardiac injury .
Recombinant human cardiac troponin complexes are produced using recombinant DNA technology. This involves inserting the genes encoding the troponin subunits into a host organism, such as bacteria or yeast, which then produces the proteins. These recombinant proteins are used in research and clinical diagnostics to ensure consistency and reliability in troponin assays .
- Standardization of Assays: Recombinant troponin complexes are used as reference materials to standardize troponin assays across different laboratories. This helps in reducing variability and improving the accuracy of diagnostic tests .
- Development of Therapeutics: Understanding the structure and function of the troponin complex can aid in the development of new therapies for heart diseases. Researchers use recombinant troponin to study the molecular mechanisms of muscle contraction and identify potential drug targets .
- Biomarker Discovery: Recombinant troponin is used in the discovery and validation of new biomarkers for cardiac diseases. It provides a reliable source of protein for experimental studies and clinical trials .
