Treponema p47 47.7kDa
Sterile Filtered solution.
Description
Recombinant Treponema pallidum p47 derived from full length TPN47 gene is fused to a 6xHis tag at C-terminus. The total Mw is 47kDa and the pI is 5.86, was purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Treponema pallidum is a spirochete bacterium responsible for syphilis, a sexually transmitted infection. The p47 protein, also known as the 47 kDa lipoprotein, is one of the major antigens of Treponema pallidum. Recombinant versions of this protein are used in various research and diagnostic applications.
The recombinant Treponema pallidum p47 protein is typically expressed in Escherichia coli systems. The protein is often fused to a 6xHis tag at the C-terminus to facilitate purification . The molecular weight of the recombinant protein is approximately 47 kDa, and it is purified using chromatographic techniques .
The p47 protein contains immunodominant regions that are recognized by the immune system of individuals infected with Treponema pallidum. Specifically, the recombinant protein includes amino acids 33-375 of the native p47 protein . This region is crucial for its immunoreactivity and is often used in ELISA and Western blot assays to detect antibodies against Treponema pallidum .
Recombinant p47 protein is widely used in research and diagnostic applications. It is suitable for use in ELISA and Western blot assays to detect antibodies in the sera of infected individuals . The protein’s high purity and specific immunoreactivity make it a valuable tool for studying the immune response to Treponema pallidum and for developing diagnostic tests.
The recombinant p47 protein is typically supplied in a buffer containing sodium phosphate, imidazole, sodium chloride, and glycerol . It should be stored at -20°C to maintain its stability and avoid freeze/thaw cycles. Under these conditions, the protein has a shelf life of approximately six months .
