T.pallidum p47 (Partial)
Treponema pallidum p47 (Partial) Recombinant
Cat. No.
BT8993
Source
Escherichia Coli.
Synonyms
Appearance
Purity
Treponema Pallidum protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
The E.Coli derived recombinant protein is fused at the C- terminus with a 6xHis Tag and contains the T.Pallidum p47 immunodominant regions.
Product Specs
Introduction
Treponema pallidum, a gram-negative spirochete bacterium with a helical structure, is known for its limited metabolic capabilities. This bacterium, categorized as metabolically crippled, has at least four subspecies: T. pallidum pallidum, T. pallidum pertenue, T. pallidum carateum, and T. pallidum endemicum. Its helical shape facilitates corkscrew-like movement through viscous substances like mucus. Notably, T. pallidum pallidum possesses a remarkably small bacterial genome, comprising 1.14 million base pairs, which underscores its adaptation to the nutrient-rich environment of mammalian tissue.
Description
This recombinant protein, derived from E. coli, is characterized by a C-terminal fusion with a 6xHis Tag. It encompasses the immunodominant regions of the T. pallidum p47 protein.
Purity
The purity of the Treponema Pallidum protein exceeds 95%, as determined by 10% PAGE (Coomassie staining).
Formulation
The protein is supplied in a buffer solution containing 70mM Tris-HCl (pH 8.0), 50mM NaCl, 50% Glycerol, and 1.5M urea.
Stability
For optimal storage, Treponema Pallidum protein should be kept at temperatures below -18°C. While it can remain stable at 4°C for up to one week, it is advisable to avoid repeated freeze-thaw cycles.
Applications
The Treponema Pallidum protein is a versatile reagent suitable for use in ELISA and Western blot assays. It serves as an excellent antigen for the specific detection of T. pallidum, minimizing potential specificity issues.
Source
Escherichia Coli.
Purification Method
Treponema Pallidum protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of T.Pallidum infected individuals.
Product Science Overview
Structure and Function
The p47 protein is a 47 kDa lipoprotein that serves as a major integral membrane immunogen. It is expressed in Escherichia coli and is often used in research for its immunogenic properties . The protein is involved in several critical functions:
- Penicillin Binding: Tp47 binds penicillin covalently in a zinc-dependent manner, although this binding does not require lipidation .
- Carboxypeptidase Activity: It exhibits zinc-dependent carboxypeptidase activity, which may help decrease cross-linking of peptidoglycan, promoting the bacterium’s motility .
- Immunogenicity: The protein contains immunodominant regions that are reactive with sera from individuals infected with Treponema pallidum .
Recombinant Production
Recombinant Tp47 is produced by expressing the protein in Escherichia coli. The recombinant form is typically purified to a high degree (over 90% purity) and is used in various applications such as ELISA and Western Blotting . The recombinant protein often includes a His-tag for easier purification and detection .
Applications in Research
The recombinant Tp47 protein is valuable in research for several reasons:
- Diagnostic Tool: It is used in ELISA tests to detect antibodies against Treponema pallidum in patient sera, aiding in the diagnosis of syphilis .
- Vaccine Development: Due to its immunogenic properties, Tp47 is studied as a potential candidate for vaccine development against syphilis .
- Pathogenesis Studies: Researchers use Tp47 to understand the pathogenesis of Treponema pallidum and its interaction with the host immune system .
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