TRAPPC2 Human

Trafficking Protein Particle Complex 2 Human Recombinant
Cat. No.
BT13998
Source
Escherichia Coli.
Synonyms
Trafficking Protein Particle Complex 2, Sedlin, SEDL, Trafficking Protein Particle Complex Subunit 2, Spondyloepiphyseal Dysplasia Late, TRAPPC2P1, HYP38334, ZNF547L, MIP2A, TRS20, SEDT, TRAPPC2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TRAPPC2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 163 amino acids (1-140 a.a.) and having a molecular mass of 18.8kDa.
TRAPPC2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Trafficking Protein Particle Complex 2 (TRAPPC2) is believed to play a role in the intricate process of transporting cargo within cells. It is thought to be part of a larger complex that helps move vesicles, small sacs that carry molecules, from the endoplasmic reticulum (where proteins are made) to the Golgi apparatus (where proteins are modified and sorted). This protein may also interact with other proteins involved in controlling gene activity. Mutations in the TRAPPC2 gene can lead to a skeletal disorder called spondyloepiphyseal dysplasia tarda (SEDT).
Description
This product consists of the human TRAPPC2 protein, manufactured in a lab using E. coli bacteria. It is a single chain of 163 amino acids, with a molecular weight of 18.8kDa. The protein is not glycosylated, meaning it does not have sugar molecules attached. For purification and detection purposes, a 23 amino acid His-tag is attached to its N-terminus.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
This solution contains 0.25mg/ml of TRAPPC2 protein in a buffer composed of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 40% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), keep the solution refrigerated at 4°C. For longer periods, freeze the solution at -20°C. Adding a carrier protein (like HSA or BSA) at a concentration of 0.1% is recommended for long-term storage. Avoid repeatedly freezing and thawing the solution.
Purity
The purity of this product is over 90%, as assessed by SDS-PAGE analysis.
Synonyms
Trafficking Protein Particle Complex 2, Sedlin, SEDL, Trafficking Protein Particle Complex Subunit 2, Spondyloepiphyseal Dysplasia Late, TRAPPC2P1, HYP38334, ZNF547L, MIP2A, TRS20, SEDT, TRAPPC2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSGSFYF VIVGHHDNPV FEMEFLPAGK AESKDDHRHL NQFIAHAALD LVDENMWLSN NMYLKTVDKF NEWFVSAFVT AGHMRFIMLH DIRQEDGIKN FFTDVYDLYI KFSMNPFYEP NSPIRSSAFD RKVQFLGKKH LLS.

Product Science Overview

Introduction

Trafficking Protein Particle Complex 2 (TRAPPC2), also known as Sedlin, is a crucial component of the trafficking protein particle (TRAPP) complex. This complex plays a significant role in the transport of proteins between cellular compartments, particularly between the endoplasmic reticulum (ER) and the Golgi apparatus .

Function and Importance

TRAPPC2 is involved in the transport of large proteins, such as procollagens, from the ER to the Golgi apparatus. This transport is essential for the proper processing and modification of these proteins, which are later transformed into mature collagen. Collagen is vital for the strength and support of connective tissues, including skin, bone, cartilage, tendons, and ligaments .

Genetic Implications

Mutations in the TRAPPC2 gene can lead to X-linked spondyloepiphyseal dysplasia tarda, a condition that impairs bone growth and predominantly affects males. This condition usually manifests between the ages of 6 and 10 and is characterized by a lack of functional Sedlin protein, which disrupts the transport of procollagen and reduces the amount of mature collagen in cells .

Preparation Methods

The preparation of recombinant TRAPPC2 involves the expression of the protein in a suitable host, such as HEK293T cells. The recombinant protein is then purified using affinity chromatography techniques. The protein is typically stored at -80°C to maintain its stability and avoid repeated freeze-thaw cycles .

Synthetic Routes

The synthetic routes for TRAPPC2 involve the use of cDNA clones or amino acid sequences to express the protein in host cells. The protein is then captured through anti-DDK affinity columns followed by conventional chromatography steps to ensure high purity and concentration .

Chemical Reactions Analysis

TRAPPC2 is part of the TRAPP complex, which regulates multiple membrane trafficking pathways. The TRAPP complexes exist in three forms, each activating specific GTPases such as Ypt1 or RAB1. These complexes play a crucial role in the secretory pathway, ensuring the directionality and fidelity of protein transport from the ER to the Golgi complex and beyond .

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Source
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