TPO Human, His

Thrombopoietin Human Recombinant, His Tag
Cat. No.
BT22657
Source
Sf9, Insect cell.
Synonyms
MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin Nirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth and Development Factor, Megakaryocyte Colony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte Stimulating Factor, Prepro-Thrombopoietin.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TPO produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (22-353aa.) and having a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57KDa).
TPO is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques..

Product Specs

Introduction
Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.
Description
TPO produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (22-353aa.) and having a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57KDa). TPO is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered clear solution.
Formulation
TPO protein solution (0.25mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Synonyms
MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin Nirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth and Development Factor, Megakaryocyte Colony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte Stimulating Factor, Prepro-Thrombopoietin.
Source
Sf9, Insect cell.
Amino Acid Sequence
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG
LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH.

Product Science Overview

Structure and Function

Thrombopoietin is encoded by the THPO gene and consists of 353 amino acids. The recombinant form of human thrombopoietin, often produced in HEK293 cells, includes a His-tag (a sequence of histidine residues) at the C-terminus. This His-tag facilitates the purification process through affinity chromatography .

Biological Activity

Recombinant human thrombopoietin (rhTPO) has been extensively studied for its ability to stimulate megakaryocyte growth and platelet production. It is biologically active in reducing thrombocytopenia (a condition characterized by low platelet counts) induced by nonmyeloablative chemotherapy . However, its benefits in stem cell transplantation or leukemia chemotherapy have not been demonstrated .

Clinical Applications

Thrombopoietin has been investigated for various clinical applications, including:

  • Chemotherapy-induced thrombocytopenia: rhTPO has shown promise in reducing thrombocytopenia associated with chemotherapy .
  • Chronic thrombocytopenia: Studies have explored its use in treating chronic thrombocytopenia related to myelodysplastic syndromes, idiopathic thrombocytopenic purpura, HIV, and liver disease .
  • Surgical thrombocytopenia: Animal studies suggest that TPO may be effective in reducing surgical thrombocytopenia and bleeding .
Production and Purification

The recombinant form of TPO is produced using mammalian cell lines, such as HEK293 cells. The His-tagged version allows for efficient purification using nickel affinity chromatography, ensuring high purity and activity of the protein .

Future Directions

Ongoing and future studies aim to further define the clinical role of recombinant TPO and TPO mimetics in the treatment of various forms of thrombocytopenia. Researchers are also exploring its potential in ex vivo expansion of pluripotent stem cells and as a radioprotectant .

In summary, Thrombopoietin (Human Recombinant, His Tag) is a vital protein with significant potential in clinical applications related to platelet production and thrombocytopenia management. Its recombinant form, enhanced with a His-tag, allows for efficient production and purification, making it a valuable tool in both research and therapeutic settings.

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