TNNI3 Human, His
Description
Recombinant Human TNNI3 protein produced in E.Coli is a full length protein containing a total of 260 amino acids and fused to a 6 His Tag at C-terminus, migrating as a 27kDa band on SDS-PAGE.
Purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Cardiac Troponin-I (cTnI) is a critical protein in the regulation of cardiac muscle contraction. It is part of the troponin complex, which includes Troponin T (TnT) and Troponin C (TnC). This complex plays a pivotal role in the calcium-mediated regulation of muscle contraction in the heart. The recombinant form of Cardiac Troponin-I, tagged with a histidine (His) sequence, is widely used in research and diagnostic applications.
Cardiac Troponin-I is a 209 amino acid protein with a molecular weight of approximately 23.9 kDa . It is unique among the troponins due to its N-terminal extension of 26 amino acids . This protein is specifically expressed in cardiac muscle tissue and is involved in inhibiting the actomyosin ATPase activity in the absence of calcium, thereby preventing muscle contraction.
The His tag, a sequence of histidine residues, is added to the recombinant protein to facilitate its purification and detection. This tag binds strongly to nickel ions, allowing for easy separation of the recombinant protein from other cellular components using affinity chromatography.
- Diagnostic Marker: Cardiac Troponin-I is a highly sensitive and specific marker for myocardial damage. Elevated levels of cTnI in the serum are indicative of myocardial infarction (heart attack) or other forms of cardiac injury .
- Research: The recombinant form of cTnI is used in various research applications to study cardiac muscle physiology, the mechanisms of muscle contraction, and the effects of different drugs on cardiac function .
- Drug Development: It is also used in high-throughput screening assays to identify potential therapeutic compounds that can modulate cardiac muscle contraction .
Recombinant Cardiac Troponin-I is stable for up to six months when stored at temperatures lower than -70°C . Upon reconstitution, it remains stable for up to one week at 4°C or up to three months at -20°C. For long-term storage, it is recommended to add a carrier protein, such as 0.1% Bovine Serum Albumin (BSA), to prevent degradation .
