TNNI3 Paired Antibody
Description
TNNI3 Paired antibodies, coating and conjugating are used for lateral flow immunoassay.
Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibodies (100µg in total).
Product Specs
This product contains two antibodies designed to work together in a lateral flow immunoassay to detect the presence of TNNI3. One antibody is designed to bind to a surface (coating antibody), while the other is designed to be linked to a detectable label (conjugating antibody).
Important: When ordering, for example, 100µg of antibody, you will receive two vials, each containing 50µg of one of the antibodies, totaling 100µg.
The Cardiac Troponin-I coating antibody solution (5mg/ml concentration) is formulated in a solution containing 50 mM Na-citrate (pH 6.0), 0.9% NaCl, and 0.095% NaN3 (as a preservative).
The Cardiac Troponin-I conjugating antibody solution (1mg/ml concentration) is formulated in a solution containing 50 mM Na-citrate (pH 6.0), 0.9% NaCl, and 0.095% NaN3 (as a preservative).
These antibodies are designed for use in lateral flow immunoassays.
Purified monoclonal IgG by protein A chromatography.
Product Science Overview
Troponin I (TnI) is a critical component of the troponin complex, which also includes troponin T (TnT) and troponin C (TnC). This complex is essential for the regulation of muscle contraction in striated muscles, including cardiac muscle. The TnI subfamily consists of three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TNNI3 gene encodes the cardiac-specific isoform of TnI, known as cardiac troponin I (cTnI), which is exclusively expressed in cardiac muscle tissues .
Cardiac troponin I (cTnI) plays a pivotal role in the regulation of cardiac muscle contraction. It acts as the inhibitory subunit by blocking actin-myosin interactions, thereby mediating muscle relaxation. Mutations in the TNNI3 gene can lead to various cardiac conditions, including familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM) .
- Lateral Flow Immunoassay: The paired antibodies are used for coating and conjugating in lateral flow immunoassays. These assays are commonly used for point-of-care testing to quickly and accurately measure cTnI levels in blood samples .
- ELISA: The Mouse Anti-Human Troponin I Type 3 Paired Antibody functions as a capture antibody in ELISA assays. It is paired with a biotinylated detection antibody to quantify cTnI levels in various assay platforms .
The antibody is typically supplied as a lyophilized product or in a sterile filtered solution. It should be stored at -20 to -70°C for long-term storage and is stable for up to 12 months under these conditions. Reconstituted antibodies should be stored at 2 to 8°C and are stable for up to one month .
