TNNI3 Human Chimeric
Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
Description
TNNI3 Human Chimeric produced in E.Coli is a single, non-glycosylated polypeptide chain (28-110 a.a.) and having a molecular mass of 29072 Dalton.
Product Specs
Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817.
Product Science Overview
Cardiac Troponin-I (cTnI) is a critical component of the troponin complex, which plays a pivotal role in the regulation of cardiac muscle contraction. The troponin complex consists of three subunits: Troponin I (cTnI), Troponin T (cTnT), and Troponin C (cTnC). Among these, cTnI is unique to cardiac muscle and serves as a highly specific biomarker for myocardial injury, particularly in the diagnosis of acute myocardial infarction (AMI) .
The development of human recombinant cTnI involves the use of recombinant DNA technology to produce a synthetic version of the protein. This process typically includes the following steps:
- Gene Cloning: The gene encoding human cTnI is isolated and inserted into a suitable expression vector.
- Transformation: The vector is introduced into a host cell, often Escherichia coli or yeast, which will express the recombinant protein.
- Protein Expression: The host cells are cultured under conditions that promote the expression of cTnI.
- Purification: The recombinant cTnI is extracted and purified using techniques such as affinity chromatography.
The resulting human recombinant cTnI is used in various clinical assays to measure troponin levels in patients’ blood samples, aiding in the diagnosis and management of cardiac conditions .
Human recombinant cTnI is crucial for standardizing clinical assays, ensuring consistency and accuracy across different laboratories and testing platforms. The use of recombinant cTnI helps mitigate the variability observed in traditional assays, which can differ significantly in their sensitivity and specificity .
