Introduction
TNFR2, a member of the TNF-receptor superfamily, exhibits a high affinity for TNFSF2/TNF-alpha and a five-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. This receptor plays a crucial role in mediating the metabolic effects of TNF-alpha. Studies conducted on knockout mice suggest that TNFR2 protects neurons from apoptosis by activating antioxidative pathways. Furthermore, TNFR2 expression may be implicated in angiogenesis, tumor cell proliferation, and metastasis in Invasive micropapillary carcinoma of the breast. Soluble TNF receptors, namely sTNFR-I and sTNFR-II, neutralize the biological activities of TNF alpha and TNF beta. These soluble receptors are believed to be generated through the shedding of the extracellular domains of their membrane-bound counterparts. Elevated levels of soluble TNF receptors have been observed in the amniotic fluid of pregnant women. TNFR2 and TNFR1 interact to form a heterocomplex that facilitates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, both of which possess E3 ubiquitin ligase activity. Although the precise function of IAPs in TNF-receptor signaling remains elusive, c-IAP1 is thought to enhance TNF-induced apoptosis by ubiquitinating and degrading TNF-receptor-associated factor 2, a mediator of anti-apoptotic signals. Oxidative stress has been shown to promote TNFR1 and TNFR2 self-interaction, leading to ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1, and TNFR2 play important roles in cellular differentiation and cell type-specific renal injury.
Description
TNFR2, a product of Sf9 Baculovirus cell expression, is a single, glycosylated polypeptide chain. This protein consists of amino acids 23-257 of TNFR2 fused to a C-terminal 6 aa His Tag, resulting in a total of 241 amino acids and a molecular mass of 25.9 kDa. SDS-PAGE analysis under reducing conditions reveals multiple bands for TNFR2 between 28-40 kDa. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The TNFR2 protein solution is provided at a concentration of 1 mg/ml in a buffer consisting of Phosphate buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the TNFR2 protein should be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity of TNFR2 is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Tumor Necrosis Factor Receptor Superfamily, Member 1B, TNFR2, TNFBR, Tumor Necrosis Factor Receptor Type II, Tumor Necrosis Factor Receptor 2, P80 TNF-Alpha Receptor, TNF-RII, TNF-R2, P75, Tumor Necrosis Factor Receptor Superfamily Member 1B, Tumor Necrosis Factor Binding Protein 2, Tumor Necrosis Factor Beta Receptor, Soluble TNFR1B Variant 1, P75 TNF Receptor, CD120b Antigen, Etanercept, TNF-R-II, TNF-R75, P75TNFR, TNFR-II, CD120b, TNFR1B, TNFR80, TBPII.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
LPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPTRSMAPG AVHLPQPVST RSQHTQPTPE PSTAPSTSFL LPMGPSPPAE GSTGDHHHHH H
