TNFR2 Human Fc

Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant
Cat. No.
BT26861
Source
Chinese Hamster Ovarian Cells (CHO).
Synonyms
Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(c) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilo Dalton (p75) tumor necrosis factor receptor 2 (TNFR2) linked to the Fc portion of human IgG1. The Fc component of TNFR2 contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilo Daltons.
The TNFR2 is purified by standard chromatographic techniques.

Product Specs

Introduction
Tumor Necrosis Factor Receptor 2 (TNFR2), also known as TNFRSF1B, is a receptor for Tumor Necrosis Factor (TNF). TNFR2 plays a crucial role in inflammatory and immune responses, particularly in conditions like rheumatoid arthritis, juvenile rheumatoid arthritis, ankylosing spondylitis, and plaque psoriasis. TNF, a naturally occurring cytokine, is involved in these inflammatory processes, and elevated levels are found in affected tissues and fluids. TNFR2 exists in both membrane-bound and soluble forms and is responsible for binding TNF and mediating its biological effects. This involvement in inflammatory pathways makes TNFR2 a potential therapeutic target for these conditions.
Description
Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein is a laboratory-produced protein designed to mimic the structure and function of the naturally occurring human TNFR2. This specific product is a dimer, meaning it consists of two identical molecules linked together. It is glycosylated, indicating the presence of sugar molecules attached to the protein, which is a common modification in proteins. The structure includes the extracellular portion of TNFR2, responsible for binding TNF, fused with the Fc region of human IgG1, an antibody. This Fc region enhances the protein's stability and lifespan in circulation. The protein is produced in CHO cells, a commonly used mammalian cell line for protein production.
Physical Appearance
The product appears as a sterile, white powder after freeze-drying.
Formulation
For every milligram of the product, it contains 1.6 milligrams of mannitol, 0.4 milligrams of sucrose, and 48 micrograms of tromethamine. These ingredients serve as stabilizers and buffering agents to maintain the protein's integrity and activity.
Solubility
To prepare the product for use, dissolve the freeze-dried powder in sterile water for injection (18 megaohm-centimeter purity) at a minimum concentration of 100 micrograms per milliliter. This reconstituted solution can then be diluted further into other aqueous solutions as needed.
Stability
To ensure product stability: Store the lyophilized powder below -18 degrees Celsius. It remains stable at room temperature for up to 3 weeks. After reconstitution, store at 4 degrees Celsius for a maximum of 7 days. For long-term storage, freeze the reconstituted solution below -18 degrees Celsius, adding a carrier protein like HSA or BSA (0.1%) for optimal preservation. Avoid repeated freezing and thawing cycles to maintain protein activity.
Purity
The purity of this product is greater than 98%, determined by rigorous analytical techniques: High-Performance Liquid Chromatography (RP-HPLC) and SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).
Biological Activity
The biological activity is measured by the protein's ability to neutralize TNF-alpha and prevent it from inhibiting the growth of A375 cells. This product has a Specific Activity of 17,000,000 IU/mg.
Synonyms
Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.
Source
Chinese Hamster Ovarian Cells (CHO).

Product Science Overview

Introduction

Tumor Necrosis Factor Receptor 2 (TNFR2) is a member of the tumor necrosis factor receptor superfamily, which plays a crucial role in the regulation of immune responses and inflammation. The fusion protein of TNFR2 with the Fc region of human immunoglobulin G1 (IgG1) is a recombinant therapeutic protein designed to modulate the activity of tumor necrosis factor-alpha (TNF-α), a pro-inflammatory cytokine involved in various autoimmune and inflammatory diseases.

Structure and Mechanism of Action

The TNFR2 fusion protein, often referred to as Etanercept or by its biosimilar names, consists of the extracellular ligand-binding domain of TNFR2 linked to the Fc portion of human IgG1. This fusion enhances the stability and half-life of the protein in the bloodstream. The primary mechanism of action involves the binding of the fusion protein to TNF-α, thereby preventing TNF-α from interacting with its natural receptors on cell surfaces. This inhibition reduces the inflammatory response mediated by TNF-α.

Clinical Applications

The TNFR2 fusion protein has been extensively studied and utilized in the treatment of several autoimmune and inflammatory conditions, including:

  • Rheumatoid Arthritis (RA): Clinical trials have demonstrated the efficacy of TNFR2 fusion protein in reducing the symptoms and progression of RA. It is often used in combination with methotrexate to enhance therapeutic outcomes .
  • Psoriasis: The fusion protein has shown significant efficacy in treating moderate-to-severe plaque psoriasis, improving skin lesions and reducing inflammation .
  • Ankylosing Spondylitis: Patients with ankylosing spondylitis have also benefited from the anti-inflammatory effects of the TNFR2 fusion protein.
Safety and Efficacy

Clinical studies have consistently shown that the TNFR2 fusion protein is effective in managing symptoms of autoimmune diseases with an acceptable safety profile. Common adverse events include upper respiratory tract infections and abnormal liver function tests . The combination therapy with methotrexate has been particularly effective in achieving higher response rates and better clinical outcomes .

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