TNFA Rat, His Active
Tumor Necrosis Factor-alpha, TNF a His, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
TNFA Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids (80-235 a.a) and having a molecular mass of 19.9kDa.
TNFA Rat is expressed with an 25 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Tumor necrosis factor (TNF), a cytokine, plays a crucial role in systemic inflammation and belongs to a group of cytokines that trigger the acute phase reaction. Primarily secreted by macrophages, TNF exerts various effects, including apoptotic cell death, cellular proliferation and differentiation, inflammation, tumorigenesis, and viral replication. Moreover, it participates in lipid metabolism and coagulation. The regulation of immune cells constitutes the primary role of TNF. Notably, dysregulation, particularly overproduction, of TNF has been linked to various human diseases, including autoimmune disorders, insulin resistance, and cancer.
Recombinant Rat TNFA, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 181 amino acids (spanning positions 80-235). With a molecular mass of 19.9 kDa, this TNFA variant is expressed with a 25 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The TNFA Rat protein solution is provided at a concentration of 1 mg/ml and contains Phosphate Buffered Saline (pH 7.4) as the buffer, along with 10% glycerol and 1 mM DTT as stabilizing agents.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the product should be avoided.
The purity of the protein is determined to be greater than 90.0% using SDS-PAGE analysis.
The biological activity of TNFA Rat is evaluated using a cytotoxicity assay with L929 mouse fibrosarcoma cells in the presence of actinomycin D, a metabolic inhibitor. The ED50, representing the effective concentration that induces a 50% response, is determined to be less than or equal to 0.2 ng/ml.
Tumor Necrosis Factor-alpha, TNF a His, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.
MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L.
Product Science Overview
Tumor Necrosis Factor-alpha (TNF-α) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. TNF-α is primarily secreted by macrophages and can induce cell death in certain tumor cell lines. The recombinant form of TNF-α, particularly the rat version with a His tag, is widely used in research to study its structure, signaling pathways, and diverse functions.
The recombinant TNF-α protein is engineered to include a His tag, which is a series of histidine residues added to the protein’s N- or C-terminus. This tag facilitates the purification of the protein using metal affinity chromatography. The rat TNF-α recombinant protein is typically expressed in E. coli, ensuring high yield and purity. The His tag does not interfere with the protein’s biological activity, making it a valuable tool for research.
TNF-α plays a crucial role in various physiological and pathological processes, including:
- Inflammation: TNF-α is a key mediator of inflammation, promoting the recruitment of immune cells to sites of infection or injury.
- Apoptosis: It can induce programmed cell death in certain cell types, contributing to the elimination of cancerous or infected cells.
- Cell Proliferation and Differentiation: TNF-α influences the growth and differentiation of various cell types, including immune cells.
- Metabolism: It is involved in lipid metabolism and can affect insulin resistance, linking it to metabolic disorders.
The rat recombinant TNF-α with a His tag is used in various research applications:
- Signaling Pathways: Researchers study TNF-α signaling pathways to understand its role in immune responses and disease mechanisms.
- Immune Cell Functions: It is used to investigate how TNF-α affects the functions of different immune cells, such as macrophages and T cells.
- Disease Pathogenesis: TNF-α is implicated in several diseases, including autoimmune disorders, cancer, and metabolic diseases. Studying its effects can provide insights into disease mechanisms and potential therapeutic targets.
