TNFA Mouse, Sf9

Tumor necrosis factor (TNF) is a cytokine that plays a crucial role in systemic inflammation and belongs to a group of cytokines responsible for stimulating the acute phase reaction. Primarily secreted by macrophages, TNF exerts various biological effects, including apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. Furthermore, it participates in lipid metabolism and coagulation processes. The primary function of TNF lies in regulating immune cells. Notably, dysregulation and excessive production of TNF have been implicated in a range of human diseases, including autoimmune disorders, insulin resistance, and cancer.

Recombinant Mouse TNFA, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 162 amino acids (80-235 a.a.). With a molecular mass of 18 kDa, it appears as a band at approximately 18-28 kDa on SDS-PAGE. This TNFA protein is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Sterile, colorless solution.

TNFA protein solution at a concentration of 1 mg/ml in phosphate-buffered saline (pH 7.4).

For short-term storage (up to 2-4 weeks), store at 4°C. For longer periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity greater than 90.0% as determined by SDS-PAGE analysis.

Tnfa, Tnfsf2, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2.

Sf9, Baculovirus cells.

LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HH