TNF a Rabbit
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The TNF-alpha is purified by standard chromatographic techniques.
Product Specs
The ED50, which represents the effective concentration required to achieve 50% of the maximum response, is determined by measuring the cytolysis (cell death) of murine L929 cells in the presence of Actinomycin D. The ED50 is less than 0.03ng/ml. This corresponds to a Specific Activity of 30,000,000 IU/mg.
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2.
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg.
Product Science Overview
Tumor Necrosis Factor-Alpha (TNF-α) is a cytokine involved in systemic inflammation and is part of the body’s immune response. It is a member of the TNF superfamily, which consists of various proteins that can cause cell death (apoptosis). TNF-α is produced mainly by macrophages, but it can also be produced by other cell types such as lymphocytes, natural killer cells, and neurons.
Recombinant Rabbit TNF-α is produced using an E. coli expression system. The target gene encoding TNF-α is expressed, resulting in a protein that consists of three identical polypeptide chains, each containing 158 amino acids. These chains combine to form a compact, bell-shaped homotrimer . The molecular mass of the individual subunit is approximately 17.4 kDa .
TNF-α is a pleiotropic cytokine, meaning it has multiple effects on different types of cells. It functions by binding to two types of receptors: TNF-R1 (TNF receptor type 1, p55R) and TNF-R2 (TNF receptor type 2, p75R) . The binding of TNF-α to these receptors can lead to various cellular responses, including:
- Promotion of inflammation: TNF-α is a potent inflammatory cytokine that can induce the production of other pro-inflammatory cytokines, such as interleukin-1 (IL-1) and interleukin-6 (IL-6).
- Induction of apoptotic cell death: TNF-α can trigger apoptosis in certain cell types, which is a crucial mechanism for eliminating cancerous or infected cells.
- Inhibition of tumorigenesis: TNF-α has anti-tumor properties and can inhibit the growth and spread of tumors.
- Inhibition of viral replication: TNF-α can interfere with the replication of certain viruses, thereby helping to control viral infections .
Recombinant Rabbit TNF-α is widely used in research to study its various biological functions and its role in diseases. Some of the key applications include:
- Cancer research: TNF-α is studied for its potential use in cancer therapy due to its ability to induce apoptosis in tumor cells.
- Inflammatory diseases: Researchers investigate the role of TNF-α in inflammatory diseases such as rheumatoid arthritis, Crohn’s disease, and psoriasis.
- Infectious diseases: TNF-α is studied for its role in the immune response to infections, particularly in the context of viral infections .
Recombinant Rabbit TNF-α is typically lyophilized and should be stored at temperatures below -20°C for long-term stability. Once reconstituted, the protein solution can be stored at 2-8°C for short-term use (2-7 days) or at -20°C for longer periods (up to 3 months). It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .
