Tumor Necrosis Factor-alpha (TNF-α) is a potent pro-inflammatory cytokine that plays a crucial role in the immune system. It is involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. TNF-α is produced primarily by macrophages, but it can also be produced by other cell types such as lymphocytes, natural killer cells, and neurons.
The human recombinant TNF-α produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 163 amino acids, with a molecular mass of approximately 18.1 kDa . The recombinant protein is expressed with a 6 amino acid His tag at the C-terminus, which facilitates its purification through chromatographic techniques .
TNF-α is a key regulator of immune cells and has a wide range of biological activities. It can induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and inhibit tumorigenesis and viral replication . The recombinant TNF-α produced in Sf9 cells retains these biological activities and is often used in research to study these processes.
Recombinant TNF-α is widely used in scientific research to study its role in various diseases, including rheumatoid arthritis, psoriatic arthritis, and psoriasis . It is also used to investigate the mechanisms of inflammation and immune response, as well as to develop and test new therapeutic agents targeting TNF-α.
The recombinant TNF-α protein is typically stored at 4°C if it will be used within 2-4 weeks. For longer storage, it is recommended to keep the protein frozen at -20°C, with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation . It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity.