TNF a Canine
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The TNF-a is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
In the context of canines, TNF-α is produced by various cells, including macrophages, monocytes, T and B cells, neutrophils, and endothelial cells . It has a key regulatory role in the canine immune response, where it modulates the expression of numerous cytokines, growth factors, inflammatory mediators, and acute phase proteins .
Recombinant Canine TNF-α is a form of TNF-α that is produced through recombinant DNA technology. This involves inserting the gene that encodes TNF-α into a host organism, such as E. coli, which then produces the protein . The recombinant protein is typically purified to high levels of purity and tested for biological activity.
Production and Purification
The recombinant canine TNF-α protein is usually expressed in E. coli and purified using methods such as High-Performance Liquid Chromatography (HPLC) and SDS-PAGE . The protein is often lyophilized and can be reconstituted in sterile PBS for use in various applications .
Applications
Recombinant canine TNF-α is used in a variety of research applications, including:
- Cytotoxicity Assays: It is used to measure the cytotoxic effects on cells, often in the presence of metabolic inhibitors like actinomycin D .
- Immunological Studies: It helps in studying the immune response and the role of TNF-α in inflammation and other immune processes .
- Drug Development: It is used in the development and testing of drugs that target TNF-α pathways, which are relevant in treating inflammatory diseases .
Stability and Storage
The stability and storage conditions for recombinant canine TNF-α are crucial for maintaining its biological activity. It is typically stored at -20 to -70°C and should be reconstituted in sterile conditions to avoid contamination .
