TIMP1 Rat
Tissue Inhibitor of Metalloprotease 1 Rat Recombinant
Cat. No.
BT10412
Source
Sf9, Baculovirus cells.
Synonyms
Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TIMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (24-217 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 200 amino acids and having a molecular mass of 22.3kDa.
TIMP1 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
TIMP1 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Introduction
TIMP1, a member of the TIMP family, is an inducible glycoprotein produced by a variety of cell types. It acts as a natural inhibitor of matrix metalloproteinases (MMPs), enzymes involved in extracellular matrix degradation. TIMP1 reversibly binds to MMPs, specifically at the substrate-binding site within the N-terminal domain. In addition to inhibiting most known MMPs, TIMP1 exhibits cell proliferation-promoting effects across various cell types and may also possess anti-apoptotic properties. Moreover, TIMP1 demonstrates erythroid-potentiating activity through nuclear translocation and inhibits apoptosis in B cells. The TIMP1 gene resides within intron 6 of the synapsin I gene, transcribed in the opposite direction. TIMP1 activity relies on the presence of disulfide bonds. Its transcription is highly inducible in response to numerous cytokines and hormones. Elevated TIMP1 levels are associated with squamous cell laryngeal carcinoma, and its overexpression is linked to gastric cancer.
Description
TIMP1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain encompassing amino acids 24-217. It is fused to a 6-amino acid His Tag at the C-terminus, resulting in a total of 200 amino acids and a molecular mass of 22.3 kDa. On SDS-PAGE under reducing conditions, TIMP1 Ligand exhibits multiple bands between 18-28 kDa. It is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
TIMP1 Ligand protein solution is supplied at a concentration of 0.5 mg/ml in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store the entire vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
CSCAPTHPQT AFCNSDLVIR AKFMGSPEII ETTLYQRYEI KMTKMLKGFD AVGNATGFRF AYTPAMESLC GYVHKSQNRS EEFLIAGRLR NGNLHITACS FLVPWHNLSP AQQKAFVKTY SAGCGVCTVF PCSAIPCKLE SDSHCLWTDQ ILMGSEKGYQ SDHFACLPRN PDLCTWQYLG VSMTRSLPLA KAEAHHHHHH
Product Science Overview
Introduction
Tissue Inhibitor of Metalloprotease 1 (TIMP-1) is a multifunctional protein that belongs to the TIMP family, which also includes TIMP-2, TIMP-3, and TIMP-4. These proteins are key regulators of matrix metalloproteinases (MMPs), enzymes responsible for the degradation and turnover of the extracellular matrix (ECM). The recombinant form of TIMP-1 derived from rats is used extensively in research to study its various biological functions and therapeutic potential.
Structure and Function
TIMP-1 is a glycoprotein that forms non-covalent complexes with active MMPs, thereby inhibiting their proteolytic activity. This inhibition is crucial for maintaining the balance between ECM synthesis and degradation, which is essential for tissue remodeling and repair. TIMP-1 specifically inhibits several MMPs, including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-14, and MMP-16 .
Biological Roles
- Regulation of ECM Turnover: TIMP-1 plays a vital role in regulating ECM turnover by inhibiting MMP activity. This regulation is crucial for processes such as wound healing, angiogenesis, and tissue remodeling.
- Cell Growth and Differentiation: TIMP-1 also functions as a growth factor that influences cell differentiation, migration, and apoptosis. It has been shown to promote cell proliferation and inhibit apoptosis in various cell types .
- Adipogenesis: Research has demonstrated that TIMP-1 negatively regulates adipogenesis. In vivo studies have shown that recombinant TIMP-1 can lead to enlarged adipocytes and impaired metabolic profiles in mice .
Therapeutic Potential
Given its role in ECM regulation and cell growth, TIMP-1 has been investigated for its therapeutic potential in various diseases:
- Cancer: TIMP-1’s ability to inhibit MMPs makes it a potential therapeutic agent in cancer, where excessive MMP activity contributes to tumor invasion and metastasis.
- Cardiovascular Diseases: TIMP-1 may have therapeutic applications in cardiovascular diseases by preventing ECM degradation and promoting tissue repair.
- Neurological Disorders: TIMP-1 has been shown to protect against blood-brain barrier disruption, suggesting potential therapeutic benefits in neurological conditions .
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