TIMP1 Human
Tissue Inhibitor of Metalloprotease 1 Human Recombinant
Cat. No.
BT9976
Source
Escherichia Coli.
Synonyms
Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
TIMP1 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 184 amino acids fragment (24-207) having a total molecular mass of 25.21kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The TIMP1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
TIMP1, a member of the TIMP family, is an inducible glycoprotein synthesized by various cell types. As a natural inhibitor of matrix metalloproteinases (peptidases involved in extracellular matrix degradation), TIMP1 reversibly binds to MMPs. Its N-terminal domain interacts with the MMP substrate-binding site. Beyond inhibiting most known MMPs, TIMP1 stimulates cell proliferation in diverse cell types and may have anti-apoptotic effects. Additionally, it exhibits erythroid-potentiating activity by translocating to the nucleus and inhibits apoptosis in B-cells. Notably, the TIMP1 gene resides within intron 6 of the synapsin I gene, transcribed in the opposite direction. Disulfide bonds are crucial for TIMP1 activity. Transcription of TIMP1 is highly inducible by various cytokines and hormones. Elevated TIMP1 levels are associated with squamous cell laryngeal carcinoma, and its overexpression is linked to gastric cancer.
Description
Recombinant human TIMP1, produced in E. coli, is a non-glycosylated polypeptide chain encompassing amino acids 24-207 (184 amino acids total). This protein, with a molecular weight of 25.21 kDa, includes a 4.5 kDa N-terminal hexahistidine tag. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
TIMP1 protein is supplied in a buffer of 25mM Sodium acetate (pH 4.8) and 50% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Repeated freezing and thawing is not recommended.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.
Source
Escherichia Coli.
Product Science Overview
Introduction
Tissue Inhibitor of Metalloprotease 1 (TIMP-1) is a multifunctional protein that belongs to the TIMP family, which also includes TIMP-2, TIMP-3, and TIMP-4 . TIMPs are naturally occurring proteins that specifically inhibit matrix metalloproteinases (MMPs), a group of enzymes involved in the degradation of the extracellular matrix (ECM) . The balance between ECM deposition and degradation is crucial for maintaining tissue homeostasis, and TIMP-1 plays a key role in this process .
Structure and Expression
Human recombinant TIMP-1 is typically expressed in Chinese Hamster Ovary (CHO) cells and is purified by substrate-affinity chromatography . The protein is largely glycosylated and has a molecular weight of approximately 29 kDa . TIMP-1 is produced in low levels in most cell types, but its production can be stimulated by treatment with phorbol ester TPA in some cell types .
Biological Functions
TIMP-1 has several biological functions, including:
- Inhibition of MMPs: TIMP-1 forms tightly bound inhibitory complexes with MMPs, thereby regulating ECM turnover and tissue remodeling .
- Angiogenesis Inhibition: TIMP-1 can inhibit angiogenesis, which is a crucial step in tumor invasion .
- Interaction with CD74: TIMP-1 interacts with CD74 to promote cell survival and proliferation .
- Ligand for Amyloid Precursor Protein: TIMP-1 acts as a novel ligand for Amyloid Precursor Protein, triggering various cellular responses .
Clinical Significance
TIMP-1 has been implicated in various physiological and pathological processes, including:
- Wound Healing: TIMP-1 plays a role in wound healing by regulating ECM degradation .
- Cancer: TIMP-1 has paradoxical effects in cancer, with both tumor-promoting and inhibitory properties . Its role in cancer is mediated by different biological functions, both MMP-dependent and MMP-independent .
- Inflammatory Diseases: TIMP-1 correlates with the progression of inflammatory diseases, including cancer .
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