C12ORF5 Human

Chromosome 12 Open Reading Frame 5 Human
Cat. No.
BT13312
Source
Escherichia Coli.
Synonyms
Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TIGAR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 270 amino acids and having a molecular mass of 30.1kDa. The TIGAR is purified by proprietary chromatographic techniques.

Product Specs

Introduction
C12ORF5, also known as TIGAR, is an enzyme involved in cellular metabolism. It plays a role in regulating glucose metabolism by acting on a molecule called fructose-2,6-bisphosphate (F-2,6-BP). F-2,6-BP is a potent activator of an enzyme crucial for glycolysis, the process of breaking down glucose for energy. TIGAR lowers the levels of F-2,6-BP within cells, which leads to a shift in glucose metabolism towards the pentose phosphate pathway. This pathway is essential for generating NADPH, a molecule important for antioxidant defense and biosynthesis.
Description
This product consists of the human TIGAR protein, produced in E. coli bacteria. It is a single chain protein, with no sugar modifications (non-glycosylated), and has a molecular weight of 30.1 kDa. The protein is purified using advanced chromatographic methods to ensure its high quality.
Physical Appearance
The product is provided as a white powder, which has been sterilized by filtration and dried by a freeze-drying process. This ensures its long-term stability and ease of handling.
Formulation
The TIGAR protein is supplied in a buffer solution containing 20mM Tris-HCl at pH 8.5 and 150mM NaCl. This solution has been filtered through a 0.2 micrometer filter to remove any particulate matter and then freeze-dried.
Solubility
To reconstitute the lyophilized TIGAR protein, it is recommended to dissolve it in sterile, ultrapure water (18 megaohm-cm resistivity) at a concentration of at least 100 micrograms per milliliter. This solution can then be further diluted as needed in other aqueous solutions.
Stability
The lyophilized TIGAR protein is stable for 3 weeks when stored at room temperature in a dry environment. For long-term storage, it is recommended to store it desiccated at -18°C or lower. Once reconstituted, the protein is stable for 2-7 days when stored at 4°C. For extended storage after reconstitution, adding a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freezing and thawing cycles to maintain protein stability.
Purity
The purity of the TIGAR protein is greater than 95%, as determined by: (a) Analysis by RP-HPLC, a high-performance liquid chromatography method; and (b) Analysis by SDS-PAGE, a gel electrophoresis technique.
Synonyms
Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.
Source
Escherichia Coli.
Amino Acid Sequence
MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII
 NFEEGREVKP TVQCICMNLQ DHLNGLTETR

Product Science Overview

Introduction

Chromosome 12 Open Reading Frame 5 (C12orf5), also known as TP53-induced glycolysis and apoptosis regulator (TIGAR), is a gene located on chromosome 12 in humans. This gene plays a crucial role in cellular metabolism and apoptosis, making it a significant subject of study in the fields of cancer research and cellular biology.

Gene Structure and Location

C12orf5 is situated on the long arm of chromosome 12 at position 12q24.11. The gene spans approximately 63.43 kilobases and consists of multiple exons and introns. The gene is known to produce several alternatively spliced mRNA variants, which lead to the production of different protein isoforms .

Expression and Regulation

The expression of C12orf5 is regulated by various factors, including the tumor suppressor protein p53. Under conditions of cellular stress, such as DNA damage, p53 activates the transcription of C12orf5. This activation leads to the production of the TIGAR protein, which plays a role in regulating glycolysis and protecting cells from oxidative stress .

Function and Mechanism

The TIGAR protein encoded by C12orf5 functions as a fructose-2,6-bisphosphatase. By decreasing the levels of fructose-2,6-bisphosphate, TIGAR reduces glycolysis and redirects glucose metabolism towards the pentose phosphate pathway. This shift helps in the generation of NADPH, which is crucial for combating oxidative stress and maintaining cellular redox balance .

Additionally, TIGAR has been implicated in the regulation of apoptosis. By modulating the levels of reactive oxygen species (ROS) within the cell, TIGAR can influence cell survival and death pathways. This dual role in metabolism and apoptosis makes TIGAR a key player in cellular homeostasis .

Clinical Significance

Given its role in regulating glycolysis and apoptosis, C12orf5/TIGAR has garnered significant attention in cancer research. Overexpression of TIGAR has been observed in various types of cancers, where it contributes to tumor growth and survival by protecting cancer cells from oxidative stress. Conversely, downregulation of TIGAR can sensitize cancer cells to chemotherapy and radiation therapy, making it a potential target for cancer treatment .

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