C12ORF5 Human, His

Chromosome 12 Open Reading Frame 5 Human Recombinant, His Tag
Cat. No.
BT13402
Source
Escherichia Coli.
Synonyms
Probable fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

C12ORF5 Human Recombinant fused with a 24 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 294 amino acids (1-270 a.a.) and having a molecular mass of 32.6kDa. The C12ORF5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The TP53-induced glycolysis and apoptosis regulator (TIGAR or C12ORF5) is a protein comprised of 270 amino acids. This protein is induced by the p53 tumor suppressor pathway and provides protection against oxidative stress. C12ORF5 specifically halts glycolysis, directing the metabolic pathway to the pentose phosphate shunt. This redirection decreases the intracellular concentration of reactive oxygen species. Therefore, it is believed that C12ORF5 might play a role in regulating the apoptotic response to p53, potentially enabling cells to withstand mild or temporary stresses.
Description
C12ORF5 Human Recombinant is a single, non-glycosylated polypeptide chain containing 294 amino acids (1-270 a.a.) and having a molecular mass of 32.6kDa. It is fused with a 24 amino acid His tag at the N-terminus and produced in E. coli. The purification of C12ORF5 is achieved using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The C12ORF5 solution (0.5 mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 2mM DTT, and 10% glycerol.
Stability
For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For extended storage periods, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Probable fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR.

Product Science Overview

Gene and Protein Structure

The C12orf5 gene is located on chromosome 12 and encodes a protein that is involved in the regulation of glycolysis and apoptosis. The protein is composed of 270 amino acids and has a molecular mass of approximately 31 kDa . The recombinant form of this protein is often produced in Escherichia coli (E. coli) and includes a C-terminal 6-His tag for purification purposes .

Function and Mechanism

TIGAR functions as a fructose-2,6-bisphosphatase, which means it can lower the levels of fructose-2,6-bisphosphate in cells. By doing so, it diverts glucose metabolism away from glycolysis and towards the pentose phosphate pathway. This shift helps in reducing reactive oxygen species (ROS) and protecting cells from oxidative stress .

Additionally, TIGAR is involved in the regulation of apoptosis. By modulating the levels of ROS, TIGAR can influence cell survival and death, making it a critical player in cellular responses to stress and damage .

Applications in Research

The recombinant form of C12orf5 (Human Recombinant, His Tag) is widely used in research to study its biochemical properties and its role in cellular metabolism and apoptosis. The His tag allows for easy purification of the protein using affinity chromatography, which is essential for obtaining high-purity samples for experimental purposes .

Researchers use this recombinant protein to investigate its enzymatic activity, interaction with other cellular components, and its overall impact on cellular physiology. Studies involving TIGAR have implications in understanding cancer metabolism, neurodegenerative diseases, and other conditions where cellular metabolism and oxidative stress play a significant role .

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