TEK Human Fc
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The TEK Fc Chimera is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
TEK Tyrosine Kinase Endothelial Fc Chimera, also known as TIE-2 or Tunica Interna Endothelial Cell Kinase, is a recombinant protein that plays a crucial role in angiogenesis, vasculogenesis, and hematopoiesis. This protein is part of the receptor tyrosine kinase (RTK) subfamily, which includes TIE-1 and TIE-2/Tek, characterized by unique structural features .
The TEK protein comprises two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region . This intricate structure allows TEK to interact with its ligands and perform its biological functions effectively.
The recombinant TEK Fc Chimera is produced in baculovirus-infected insect cells and is fused with the Fc part of human IgG1. This fusion results in a monomeric, glycosylated polypeptide containing 730 amino acids, with a total molecular mass of approximately 250 kDa . The protein is purified using proprietary chromatographic techniques to ensure high purity and functionality .
TEK Tyrosine Kinase Endothelial Fc Chimera is primarily expressed on endothelial and hematopoietic progenitor cells. It plays a critical role in:
TEK interacts with two primary ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2). Ang1 acts as an agonist, promoting blood vessel maturation and stability, while Ang2 can act as an antagonist, disrupting these processes . The balance between Ang1 and Ang2 is crucial for maintaining vascular homeostasis.
Due to its significant role in vascular biology, TEK Tyrosine Kinase Endothelial Fc Chimera is widely used in research to study:
- Vascular development and diseases: Understanding the mechanisms of blood vessel formation and identifying potential therapeutic targets for vascular diseases.
- Cancer research: Investigating the role of angiogenesis in tumor growth and metastasis.
- Drug development: Screening for potential drugs that can modulate TEK activity and its interactions with ligands .
The lyophilized TEK Fc Chimera is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent degradation, it is recommended to add a carrier protein such as 0.1% HSA or BSA and avoid repeated freeze-thaw cycles .
