TEK Mouse

TEK Tyrosine Kinase Endothelial Mouse Recombinant
Cat. No.
BT28354
Source
Sf9, Baculovirus cells.
Synonyms
Angiopoietin-1 receptor, Endothelial tyrosine kinase, HYK, STK1, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, mTIE2, p140 TEK, CD202b.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

TEK produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 730 amino acids (23-746 a.a.) and having a molecular mass of 81.6kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions).
TEK is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
The receptor tyrosine kinase (RTK) subfamily TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek is distinguished by its unusual structural features, which include two immunoglobulin-like domains that surround three epidermal growth factor (EGF)-like domains and are followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are mostly found on endothelial and hematopoietic progenitor cells, where they play important roles in angiogenesis, vasculogenesis, and hematopoiesis. Human TIE-1 cDNA encodes a precursor protein of 1122 amino acids (aa), with an 18-residue putative signal peptide, a 726-residue extracellular domain, and a 353-residue cytoplasmic domain. Two ligands that bind TIE-2 with high affinity have been found: angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2). Ang2 is thought to act as an Ang1 antagonist. Mice that have been genetically modified to overexpress Ang2 or to lack Ang1 or Tie-1 exhibit comparable angiogenic defects.
Description
TEK, a single glycosylated polypeptide chain produced in Sf9 Baculovirus cells, contains 730 amino acids (23-746 a.a.) and has a molecular mass of 81.6kDa. (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). TEK is purified using proprietary chromatographic methods and expressed with a 6 amino acid His tag at the C-Terminus.
Physical Appearance
Colorless solution that has been sterile filtered.
Formulation
TEK protein solution (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
If the entire vial will be used within 2-4 weeks, store at 4°C. For longer periods of time, store frozen at -20°C. It is advised to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Angiopoietin-1 receptor, Endothelial tyrosine kinase, HYK, STK1, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, mTIE2, p140 TEK, CD202b.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
AMDLILINSL PLVSDAETSL TCIASGWHPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGQAIR IRTMKMRQQA SFLPATLTMT VDRGDNVNIS FKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA FTRLIVRRCE AQKWGPDCSR PCTTCKNNGV CHEDTGECIC PPGFMGRTCE KACEPHTFGR TCKERCSGPE GCKSYVFCLP DPYGCSCATG WRGLQCNEAC PSGYYGPDCK LRCHCTNEEI CDRFQGCLCS QGWQGLQCEK EGRPRMTPQI EDLPDHIEVN SGKFNPICKA SGWPLPTSEE MTLVKPDGTV LQPNDFNYTD RFSVAIFTVN RVLPPDSGVW VCSVNTVAGM VEKPFNISVK VLPEPLHAPN VIDTGHNFAI INISSEPYFG DGPIKSKKLF YKPVNQAWKY IEVTNEIFTL NYLEPRTDYE LCVQLARPGE GGEGHPGPVR RFTTASIGLP PPRGLSLLPK SQTALNLTWQ PIFTNSEDEF YVEVERRSLQ TTSDQQNIKV PGNLTSVLLS NLVPREQYTV RARVNTKAQG EWSEELRAWT LSDILPPQPE NIKISNITDS TAMVSWTIVD GYSISSIIIR YKVQGKNEDQ HIDVKIKNAT VTQYQLKGLE PETTYHVDIF AENNIGSSNP AFSHELRTLP HSPASADLGG GKMLHHHHHH.

Product Science Overview

Introduction

TEK tyrosine kinase, also known as TIE2 or CD202B, is a receptor tyrosine kinase that plays a crucial role in the vascular system. It is predominantly expressed in endothelial cells, which line the interior surface of blood vessels. The TEK receptor is essential for angiogenesis, the process through which new blood vessels form from pre-existing vessels, and for maintaining the integrity of the vascular system .

Structure and Function

The TEK receptor tyrosine kinase has a unique extracellular domain that includes two immunoglobulin-like loops, three epidermal growth factor-like repeats, and three fibronectin type III-like repeats . This structure allows it to interact with its primary ligand, angiopoietin-1 (ANGPT1), which activates the receptor by inducing its tyrosine phosphorylation .

Upon activation by ANGPT1, TEK initiates a cascade of downstream signaling pathways that promote endothelial cell survival, migration, and the formation of new blood vessels . This signaling is critical for both physiological processes, such as wound healing and embryonic development, and pathological conditions, including cancer and diabetic retinopathy .

Recombinant TEK Tyrosine Kinase

Recombinant TEK tyrosine kinase refers to the protein produced through recombinant DNA technology, which involves inserting the gene encoding TEK into an expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. This recombinant protein is used in various research applications to study its function, interactions, and potential therapeutic uses.

Applications in Research
  1. Angiogenesis Studies: Recombinant TEK is used to investigate the mechanisms of angiogenesis and to identify potential therapeutic targets for diseases characterized by abnormal blood vessel formation .
  2. Cancer Research: TEK is expressed in a subpopulation of monocytes that home to tumors and are essential for the formation of new blood vessels within the tumor microenvironment. Studying recombinant TEK helps in understanding its role in tumor angiogenesis and developing anti-angiogenic therapies .
  3. Vascular Diseases: Research on recombinant TEK contributes to understanding vascular diseases, such as inherited venous malformations, where defects in TEK signaling are implicated .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

ANGPT1 Human
Angiopoietin-1 Human Recombinant
Cat. No.
BT9019
Source
E.coli.
TEK Human
TEK Tyrosine Kinase Endothelial Human Recombinant
Cat. No.
BT28441
Source
Sf9, Baculovirus cells.
TEK Human Fc
TEK Tyrosine Kinase Endothelial Fc Chimera Human Recombinant
Cat. No.
BT28524
Source
Insect Cells.
TEK Mouse Fc
TEK Tyrosine Kinase Endothelial Fc Chimera Mouse Recombinant
Cat. No.
BT28612
Source
CHO Cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.