TGFBR2 Human, His

Transforming Growth Factor Beta Receptor II, His Tag Human Recombinant
Cat. No.
BT160
Source
Sf9, Baculovirus cells.
Synonyms
AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII, TGFBR-2, TGF-beta receptor type-2, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TGF-beta type II receptor, TbetaR-II, TGFBR2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TGFBR2 Human Recombinant produced in in Sf9 Baculovirus cells is a single, non-glycosylated polypeptide chain containing 383 amino acids (23-166a.a) and having a molecular mass of 43.3kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).

TGFBR2 is expressed with a 239aa hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
TGFBR2, a member of the Ser/Thr protein kinase and TGFB receptor subfamilies, is a transmembrane protein. Upon binding TGF-beta, it forms a heterodimer with another receptor protein. This complex phosphorylates proteins, which then translocate to the nucleus and regulate the transcription of genes involved in cell proliferation. TGFBR2 mutations are implicated in Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and tumor development. Notably, TGFBR2 expression is elevated in oral squamous cell carcinoma cells but attenuated in colorectal cancer, suggesting its role in modulating TGF-beta activity. IL-1beta downregulates TGFBR2 expression while inducing Sp3 via NFkappaB. TGFBR2 and TGFB2 contribute to the antiestrogenic effects of tamoxifen metabolites in breast cancer.
Description
TGFBR2 Human Recombinant, produced in Sf9 Baculovirus cells, is a single, non-glycosylated polypeptide chain comprising 383 amino acids (23-166a.a). It has a molecular mass of 43.3kDa and appears at 40-57kDa on SDS-PAGE under reducing conditions. The protein is expressed with a 239aa hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
TGFBR2 solution (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII, TGFBR-2, TGF-beta receptor type-2, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TGF-beta type II receptor, TbetaR-II, TGFBR2.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
TIPPHVQKSV NNDMIVTDNN GAVKFPQLCK FCDVRFSTCD NQKSCMSNCS ITSICEKPQE VCVAVWRKND ENITLETVCH DPKLPYHDFI LEDAASPKCI MKEKKKPGET FFMCSCSSDE CNDNIIFSEE YNTSNPDLLL VIFQLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH.

Product Science Overview

Introduction

Transforming Growth Factor Beta Receptor II (TGFBR2) is a crucial component of the Transforming Growth Factor Beta (TGF-β) signaling pathway. This pathway plays a significant role in regulating various cellular processes, including proliferation, differentiation, and apoptosis. The recombinant form of TGFBR2, tagged with a histidine (His) tag, is commonly used in research to facilitate purification and detection.

Structure and Function

TGFBR2 is a serine/threonine kinase receptor that binds to TGF-β ligands. Upon ligand binding, TGFBR2 forms a complex with Transforming Growth Factor Beta Receptor I (TGFBR1), leading to the phosphorylation and activation of TGFBR1. This activation triggers downstream signaling cascades, primarily through the SMAD pathway, which regulates gene expression and cellular responses .

Recombinant Production

The recombinant production of TGFBR2 involves the insertion of the TGFBR2 gene into an expression vector, which is then introduced into host cells, such as E. coli or mammalian cells. The His tag is added to the N- or C-terminus of the protein to facilitate purification using immobilized metal affinity chromatography (IMAC). This method allows for the efficient isolation of the recombinant protein from the host cell lysate .

Applications in Research

Recombinant TGFBR2, His Tag (Human) is widely used in various research applications, including:

  • Structural studies: Understanding the three-dimensional structure of TGFBR2 and its interactions with ligands and other receptors.
  • Functional assays: Investigating the role of TGFBR2 in cellular signaling and its impact on cellular processes.
  • Drug discovery: Screening for potential inhibitors or modulators of TGFBR2 activity, which could be used in therapeutic interventions for diseases related to TGF-β signaling .

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Source
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