TGFBR1 Human
Sf9, Baculovirus cells.
TGFBR1, AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, tbetaR-I, TGFR-1.
Greater than 90.0% as determined by SDS-PAGE.
Description
TGFBR1produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 342 amino acids (27-126a.a.) and having a molecular mass of 38.0kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
TGFBR1is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Transforming growth factor beta receptor 1 (TGFBR1) is a single-pass transmembrane protein belonging to the TGF-beta receptor subfamily and the protein kinase superfamily. This secreted protein is involved in a wide range of cellular processes, including the regulation of cell growth, differentiation, proliferation, and programmed cell death (apoptosis). TGFBR1 also plays a role in immune system modulation and exhibits varying effects on different cell types or cells at different developmental stages.
Produced in Sf9 insect cells using a baculovirus expression system, this recombinant TGFBR1 protein is a single, glycosylated polypeptide chain consisting of 342 amino acids (27-126a.a.). It has a molecular weight of 38.0 kDa, although it may appear between 40-57 kDa on SDS-PAGE due to glycosylation. This protein is expressed with a 242 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
The TGFBR1 protein is supplied as a 0.5 mg/mL solution in phosphate-buffered saline (PBS) at pH 7.4 containing 10% glycerol.
The purity of this protein is greater than 90.0% as determined by SDS-PAGE analysis.
TGFBR1, AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, tbetaR-I, TGFR-1.
Sf9, Baculovirus cells.
ADLLLPGATA LQCFCHLCTK DNFTCVTDGL CFVSVTETTD KVIHNSMCIA EIDLIPRDRP FVCAPSSKTG SVTTTYCCNQ DHCNKIELPT TVKSSPGLGP VELVEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
Transforming Growth Factor Beta Receptor I (TGFBR1), also known as Activin Receptor-Like Kinase 5 (ALK-5), is a serine/threonine kinase receptor that belongs to the protein kinase superfamily and the TGF-beta receptor subfamily . It is a single-pass type I membrane protein that plays a crucial role in the TGF-beta signaling pathway .
TGFBR1 functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase . It is involved in the regulation of cellular growth, differentiation, apoptosis, motility, invasion, extracellular matrix production, angiogenesis, and immune responses . The receptor forms a complex with type II TGF-beta receptors upon ligand binding, leading to the phosphorylation and activation of SMAD transcriptional regulators .
Upon ligand binding, TGFBR1 forms a receptor complex consisting of two type I and two type II transmembrane serine/threonine kinases . The type II receptors phosphorylate and activate the type I receptors, which then auto-phosphorylate and bind to SMAD proteins . This activation leads to the translocation of SMAD proteins to the nucleus, where they regulate the transcription of target genes .
The TGF-beta/SMAD pathway is tightly regulated to ensure proper cellular responses. Disruption of this pathway has been implicated in various human cancers, with the majority of colon and gastric cancers being caused by inactivating mutations in TGF-beta RII . The administration of TGF-beta has been shown to protect against mammary tumor development in transgenic mouse models .
Recombinant human TGFBR1 is produced using baculovirus expression systems in insect cells . The recombinant protein is often tagged with GST (Glutathione S-transferase) to facilitate purification and detection . It is supplied in a buffer containing Tris-HCl, NaCl, glutathione, EDTA, DTT, PMSF, and glycerol to maintain stability and activity .
