TGFB2 Antibody

Transforming Growth Factor-beta 2 (TGF-β2) is a member of the TGF-β family, which plays a crucial role in regulating various cellular processes, including cell growth, differentiation, and immune responses. The TGF-β family is known for its involvement in embryonic development, tissue homeostasis, and the pathogenesis of various diseases, including cancer and fibrosis.

TGF-β2 is a multifunctional cytokine that is involved in the regulation of cell proliferation, differentiation, and apoptosis. It is produced by various cell types, including immune cells, epithelial cells, and fibroblasts. TGF-β2 signals through a receptor complex composed of type I and type II serine/threonine kinase receptors, leading to the activation of intracellular signaling pathways that modulate gene expression.

Polyclonal antibodies are produced by immunizing animals, such as rabbits, with an antigen, in this case, TGF-β2. The immune system of the rabbit generates a diverse population of antibodies that recognize multiple epitopes on the TGF-β2 protein. These antibodies are then collected from the rabbit’s serum and purified for use in various research applications.

The production of polyclonal rabbit anti-human TGF-β2 antibody involves several steps:

1. Immunization: Rabbits are immunized with a synthetic peptide corresponding to a specific region of the human TGF-β2 protein. This peptide acts as an immunogen, stimulating the rabbit’s immune system to produce antibodies against TGF-β2.
2. Serum Collection: After a series of booster immunizations, blood is collected from the rabbits, and the serum is separated from the blood cells.
3. Antibody Purification: The serum contains a mixture of antibodies, including those specific to TGF-β2. These antibodies are purified using techniques such as protein A/G affinity chromatography, which selectively binds to the Fc region of the antibodies, allowing for their isolation from other serum proteins.

The polyclonal rabbit anti-human TGF-β2 antibody is widely used in various research applications, including:

• Western Blotting: To detect and quantify TGF-β2 protein levels in cell and tissue lysates.
• Immunohistochemistry (IHC): To visualize the localization and distribution of TGF-β2 in tissue sections.
• Enzyme-Linked Immunosorbent Assay (ELISA): To measure TGF-β2 concentrations in biological samples, such as serum or cell culture supernatants.