TGFB2 Human, HEK

Transforming Growth Factor-Beta 2 Human Recombinant, HEK
Cat. No.
BT4785
Source
HEK.
Synonyms
Transforming growth factor, beta 2, cetermin, Glioblastoma-derived T-cell suppressor factor, polyergin, G-TSF, TGF-beta2, TGF-beta-2, transforming growth factor beta-2, BSC-1 cell growth inhibitor, TGFB-2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as obsereved by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

TGF-b 2 Human Recombinant produced in HEK cells is a non-glycosylated homodimer, having a total molecular weight of 25kDa.
The TGF-b 2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Transforming Growth Factor Beta 2 (TGFB2) is a protein with a molecular weight of 27.08 kDa, composed of two identical amino acid chains, each containing 118 amino acids, linked by a disulfide bond. It belongs to a family of five related cytokines that play significant roles in regulating cellular activity in various normal and cancerous cells. The three mammalian isoforms of TGF-beta, including TGFb1, TGFb2, and TGFb3, share a common signaling pathway through the same receptor and elicit similar biological responses. These isoforms are involved in essential physiological processes such as embryonic development, tissue remodeling, and wound healing.
Description
Recombinant Human TGF-b 2, produced in HEK cells, is a non-glycosylated homodimeric protein with a molecular weight of 25 kDa. The purification process of TGF-b 2 involves proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
TGF-b 2 was lyophilized from a 0.2 µm filtered solution containing 50 mM sodium acetate at a pH of 4.5.
Solubility
To reconstitute lyophilized TGF-b 2, it is recommended to use a sterile solution containing 20% ethanol, 50 mM sodium acetate, and 75 mM acetic acid.
Stability
Lyophilized TGF-b 2 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C for optimal preservation. Once reconstituted, TGF-b 2 should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to store it below -18°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity greater than 95% as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of the protein was evaluated based on its ability to inhibit IL-4-induced proliferation of mouse HT-2 cells (BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2) in a dose-dependent manner. The ED50, which represents the concentration of TGF-b 2 required for 50% inhibition, was determined to be 0.16 ng/ml.
Synonyms
Transforming growth factor, beta 2, cetermin, Glioblastoma-derived T-cell suppressor factor, polyergin, G-TSF, TGF-beta2, TGF-beta-2, transforming growth factor beta-2, BSC-1 cell growth inhibitor, TGFB-2.
Source
HEK.

Product Science Overview

Gene and Protein Structure

The TGFB2 gene is located on human chromosome 1q41 and is expressed in the extracellular matrix . The active form of TGF-β2 is produced through proteolytic cleavage of its precursor. TGF-β2 shares approximately 70% amino acid sequence identity with TGF-β1 . The recombinant form of TGF-β2, expressed in HEK 293 cells, is a non-glycosylated homodimer with a molecular weight of approximately 25 kDa .

Biological Functions

TGF-β2 is a multifunctional cytokine involved in regulating cell growth, differentiation, and survival. It plays a significant role in immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis . TGF-β2 is a potent growth inhibitor for various cell types, including epithelial, lymphoid, fibroblast, and keratinocyte cells .

Role in Disease and Therapeutics

TGF-β2 is associated with tumor development, progression, and metastasis. It acts as a tumor suppressor in the early stages of carcinogenesis but promotes tumor growth in later stages by inducing epithelial-mesenchymal transition and stimulating angiogenesis . Additionally, TGF-β2 inhibits the antitumor activity of natural killer cells, T-cells, macrophages, monocytes, and neutrophils .

TGF-β2 is also involved in inflammation and wound healing processes. Overexpression of the TGF-β gene has been observed in various cancers, including glioma, colon cancer, gastric cancer, and cervical lesions .

Applications in Research and Medicine

Recombinant TGF-β2, expressed in HEK 293 cells, is widely used in cell culture and research applications. It is essential for studying cell signaling pathways, cancer biology, and immune regulation. The recombinant form ensures high purity and activity, making it suitable for various experimental setups .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

TGFB1 Mouse
Transforming Growth Factor-Beta 1 Mouse Recombinant
Cat. No.
BT4603
Source
Escherichia Coli.
TGFB2 Human
Transforming Growth Factor Beta 2 Human Recombinant
Cat. No.
BT4700
Source
Nicotiana benthamiana.
TGFB2 Antibody
Transforming Growth Factor-beta 2 Polyclonal Rabbit Anti Human Antibody
Cat. No.
BT6036
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.