TGFB1 Human
Transforming Growth Factor-beta 1 Human
Cat. No.
BT2720
Source
Human Platelets.
Synonyms
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.
Appearance
Sterile Filtered lyophilized powder.
Purity
Greater than 98.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa.
The TGF-b 1 is purified by proprietary chromatographic techniques.
The TGF-b 1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Transforming growth factor betas (TGF-betas) are signaling molecules that play crucial roles in embryonic development by regulating cell-cell interactions. In mammals, there are three known TGF-betas: TGF-beta1, TGF-beta2, and TGF-beta3. These proteins are initially synthesized as precursor molecules. Each precursor undergoes cleavage to produce a 112-amino acid polypeptide chain that remains linked to the inactive part of the molecule.
Description
This product contains human transforming growth factor-beta 1 (TGF-beta 1), a protein with a molecular weight of 25 kDa. It is purified from human platelets using proprietary chromatography methods.
Physical Appearance
The product is a sterile, lyophilized powder that appears white in color.
Formulation
The TGF-beta1 protein was freeze-dried from a solution of 30% acetonitrile and 0.1% trifluoroacetic acid.
Solubility
To reconstitute the lyophilized TGF-beta 1, it is recommended to dissolve it in a solution of 0.5% BSA in 0.1N acetic acid. Once reconstituted, this solution can be further diluted to the desired concentration using a solution of 30% acetonitrile and 0.1% trifluoroacetic acid.
Stability
The lyophilized TGF-beta 1 remains stable for up to 3 weeks when stored at room temperature. However, it is recommended to store it in a dry environment below -18°C for optimal long-term preservation. Once reconstituted, TGF-beta 1 should be stored at 4°C for a period of 2 to 7 days. For extended storage, it should be kept below -18°C. It's important to avoid reconstituting with neutral buffers, using glass equipment, excessive handling, and repeated freeze-thaw cycles.
Purity
The purity of this product is determined to be greater than 98.0% using SDS-PAGE analysis.
Biological Activity
The biological activity of this product is measured by its ability to promote the growth of NRK-1 cells in a soft agar assay. The effective concentration range for stimulating cell growth is observed between 0.1 ng/ml to 5 ng/ml. This corresponds to a specific activity ranging from 2,000,000 to 10,000,000 IU/mg. The optimal effective concentration for your specific experiment may vary and needs to be determined empirically. It's important to note that the observation of biological activity requires the presence of purified EGF and/or TGF-.
Synonyms
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.
Source
Human Platelets.
Product Science Overview
Introduction
Gene and Protein Structure
Functions and Mechanisms
TGF-β1 is a multifunctional cytokine that performs several key functions:
- Cell Growth and Proliferation: TGF-β1 regulates the growth and proliferation of various cell types. It acts synergistically with transforming growth factor-alpha (TGF-α) in inducing cellular transformation .
- Immune System Regulation: TGF-β1 plays a significant role in controlling the immune system. It is secreted by most immune cells and can inhibit the actions of other cytokines, such as interleukin-1 (IL-1) and interleukin-2 (IL-2), thereby modulating immune responses .
- Wound Healing: TGF-β1 was first identified in human platelets and has a potential role in wound healing due to its ability to control cell growth and differentiation .
Role in Diseases
Dysregulation of TGF-β1 activation and signaling can lead to various diseases:
- Cancer: TGF-β1 acts as a tumor suppressor by inhibiting cell proliferation and inducing apoptosis. However, in tumor cells, TGF-β1 can lose its anti-proliferative response and become an oncogenic factor, promoting tumor progression .
- Fibrosis and Inflammation: Chronic overexpression of TGF-β1 is associated with diseases such as fibrosis and inflammation. It drives disease progression by modulating cell growth and migration .
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