Introduction
CCL25 (Teck), a unique CC chemokine, shares a distant relationship (approximately 20% amino acid sequence identity) with other CC chemokines. Cloning of the mouse CCL25 cDNA reveals its encoding of a 144 amino acid protein, exhibiting a 49% amino acid sequence identity to its human counterpart. Expression of both human and mouse CCL25 is largely confined to the thymus and small intestine. Dendritic cells within the thymus are identified as the primary source of CCL25 production. However, bone marrow-derived dendritic cells do not express this chemokine. CCL25, signaling through the CCR9 receptor, is believed to play a role in T-cell development. Both recombinant human and mouse Teck exhibit chemotactic activity towards activated macrophages, dendritic cells, and thymocytes. Notably, the recombinant protein demonstrates chemotactic properties on thymocytes, macrophages, THP-1 cells, and dendritic cells, while remaining inactive towards peripheral blood lymphocytes and neutrophils.
Description
Recombinant TECK Mouse, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 121 amino acids. With a molecular weight of 14.1 kDa, it is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The product is filtered (0.2 µm) and lyophilized from a concentrated solution (1 mg/ml) in 1x PBS, pH 7.4.
Solubility
To reconstitute the lyophilized TECK, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. Further dilutions can be prepared in other aqueous solutions.
Stability
Lyophilized TECK, while stable at room temperature for up to 3 weeks, should be stored desiccated at a temperature below -18°C. After reconstitution, it should be stored at 4°C for a period of 2-7 days. For extended storage, it is recommended to store it below -18°C. It is advisable to add a carrier protein (0.1% HSA or BSA) for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity is greater than 97.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity is assessed by its chemoattractant properties on human CCR9 transfected BaF3 mouse pro-B cells, using a concentration range of 0.1-0.5 µg/ml.
Synonyms
C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.
Amino Acid Sequence
QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSASDS QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N
