TECK Human, His
Description
TECK is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Thymus Expressed Chemokine (CCL25), also known as Chemokine (C-C motif) ligand 25 or TECK, is a small cytokine that belongs to the CC chemokine family. This chemokine plays a crucial role in the development of T-cells, which are essential for cell-mediated immunity. The recombinant form of CCL25, tagged with a histidine (His) sequence, is widely used in research to study its structure, function, and interactions.
The gene encoding CCL25 is located on chromosome 19 in humans. The protein consists of 150 amino acids and has a molecular mass of approximately 16,609 Daltons . Like other members of the CC chemokine family, CCL25 is characterized by two adjacent cysteines. There are three isoforms of CCL25 produced by alternative splicing . The recombinant form of CCL25, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 152 amino acids, including a 25 amino acid His-tag at the N-terminus .
CCL25 is primarily involved in the development of T-cells. It exhibits chemotactic activity, meaning it can direct the movement of certain cells, including thymocytes, macrophages, THP-1 cells, and dendritic cells . However, it is inactive on peripheral blood lymphocytes and neutrophils . CCL25 is also suspected to play a significant role in diseases such as allergic asthma and ileitis .
CCL25 exerts its effects by binding to the chemokine receptor CCR9 . This binding is crucial for the chemotactic response of thymocytes, macrophages, and dendritic cells. The CCL25/CCR9 interaction also promotes the migration and invasion of T-cells in hepatocellular and breast cancer cell lines . Additionally, CCL25 selectively enhances resistance to TNF-alpha mediated apoptosis in T-ALL and T-CLL T-cells .
The primary source of CCL25 in the thymus is a novel thymic dendritic cell-specific CC chemokine, which is potentially involved in T-cell development . Besides its chemotactic activity, CCL25 has distinctive mechanisms of lymphocyte recruitment that encourage functional specialization of immune responses in different parts of the gastrointestinal tract .
The recombinant form of CCL25, tagged with a His sequence, is used extensively in research to study its structure, function, and interactions. This form is produced in E. coli and purified using proprietary chromatographic techniques . The His-tag facilitates the purification and detection of the recombinant protein, making it a valuable tool for researchers.
