TECK Human

CCL25 is produced as a protein precursor containing 151 amino acids . The recombinant human CCL25 protein is typically expressed in E. coli and consists of 127 amino acids with a molecular weight of approximately 14.2 kDa . It is highly purified, with a purity greater than 98% as determined by SDS-PAGE .

CCL25 is primarily involved in the chemotactic response, meaning it directs the movement of immune cells towards areas where they are needed. It is chemotactic for thymocytes, macrophages, and dendritic cells . This chemokine exerts its effects by binding to the chemokine receptor CCR9 . The interaction between CCL25 and CCR9 is essential for the navigation and trafficking of developing T cells within the thymus .

In addition to its role in the thymus, CCL25 is also expressed in the small intestine, where it helps regulate the immune response in the gut . Dendritic cells in the thymus are identified as the primary source of CCL25 production, although bone marrow-derived dendritic cells do not express this chemokine .

Recombinant human CCL25 is widely used in research to study its role in immune cell trafficking and development. It is utilized in various assays, including Western blot, immunohistochemistry, ELISA, and functional assays . The activity of recombinant CCL25 is often determined by its ability to bind to the CCR9 receptor on the surface of specific cell lines, such as MOLT4 cells .

Recombinant human CCL25 is typically supplied in a lyophilized form and should be reconstituted in water to a concentration of 0.1-1.0 mg/mL . It is recommended to store the reconstituted protein at 2°C to 8°C for up to one week or at -20°C to -80°C for long-term storage . Avoid repeated freeze-thaw cycles to maintain protein stability .