ELOB Human
Description
Product Specs
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Product Science Overview
Elongin-B is a small protein with a molecular mass of approximately 13.1 kDa and consists of 118 amino acids . It is a non-glycosylated polypeptide chain produced in Escherichia coli (E. coli) and purified using standard chromatography techniques . The protein is typically stored in a sterile, filtered, colorless solution containing Tris-HCl, DTT, PMSF, and glycerol to maintain its stability .
The primary function of Elongin-B is to initiate the elongation phase of transcription by RNA polymerase II. It achieves this by suppressing the transient pausing of the polymerase at various sites within transcription units . This suppression is essential for the efficient transcription of genes and the proper regulation of gene expression.
Elongin-B forms a heterotrimeric complex with elongins A and C. This complex is known as the SIII complex and is involved in the regulation of transcription elongation . The von Hippel-Lindau (VHL) tumor suppressor protein also interacts with elongins B and C, inhibiting transcription elongation . This interaction is crucial for the regulation of gene expression and the prevention of uncontrolled cell growth, which can lead to tumor formation.
Recombinant human Elongin-B is widely used in research to study the mechanisms of transcription elongation and the regulation of gene expression. It is also used to investigate the interactions between elongins and other proteins, such as the VHL tumor suppressor protein . The recombinant protein is suitable for various applications, including SDS-PAGE and functional studies .
