ELOB Mouse
Transcription elongation factor B polypeptide 2, TCEB2, RNA polymerase IItranscription factor SIII subunit B, SIII p18, EloB, Elongin 18 kDa subunit.
Description
ELOB Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain of 141 amino acids ( 1-118 a.a.) having a molecular mass of 15.6 kDa. The Recombinant Mouse ELOB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Transcription elongation factor B polypeptide 2, TCEB2, RNA polymerase IItranscription factor SIII subunit B, SIII p18, EloB, Elongin 18 kDa subunit.
MGSSHHHHHH SSGLVPRGSH MGSMDVFLMI RRHKTTIFTD AKESSTVFEL KRIVEGILKR PPEEQRLYKD DQLLDDGKTL GECGFTSQTA RPQAPATVGL AFRADDTFEA LRIEPFSSPP ELPDVMKPQD SGGSANEQAV Q
Product Science Overview
Elongin B forms a heterodimer with Elongin C (ELOC), and together they serve as the regulatory subunits for the Elongin complex . This complex is a general transcription elongation factor that enhances the transcription process by alleviating transcriptional pausing . The Elongin B/C heterodimer also binds to the “BC-box motif” found in many proteins within the VHL-box and SOCS-box protein families .
Elongin B is also a component of Cullin-RING E3 ubiquitin ligase complexes (CRLs), specifically those based on Cullin-2 (Cul2) and Cullin-5 (Cul5) . These complexes play a central role in targeting cellular proteins for ubiquitination-dependent protein turnover through the 26S proteasome . In these complexes, Elongin B, along with Elongin C, acts as an adapter protein that helps in the assembly of the CRL complex .
The Elongin B/C complex is involved in various biological processes, including the regulation of hypoxia-inducible factors (HIFs). The most well-known CRL2 substrate recognition receptor is the tumor suppressor protein VHL (von Hippel–Lindau), which is mutated in von Hippel–Lindau syndrome, a rare hereditary cancer syndrome . The CRL2 VHL complex-dependent degradation of the α subunits of HIF (HIFα) is a critical function in tumorigenesis .
Recombinant Elongin B (Mouse) is produced using various expression systems, including baculovirus-infected insect cells. It is often used in research to study its role in transcription elongation and ubiquitination processes. The recombinant protein is typically supplied as a solution in HEPES, NaCl, DTT, and Glycerol, and it is stored at -70°C to maintain its stability .
