TCEB1 Human

Transcription Elongation Factor B Polypeptide 1 Human Recombinant
Cat. No.
BT15425
Source
Escherichia Coli.
Synonyms
Transcription elongation factor B polypeptide 1, Elongin 15 kDa subunit, Elongin-C, EloC, RNA polymerase II transcription factor SIII subunit C, SIII p15, TCEB1, SIII.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TCEB1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-112 a.a.) and having a molecular mass of 14.6kDa.
TCEB1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Elongin, a common transcription elongation factor, relies on its subunit TCEB1 (elongin C) to enhance RNA polymerase II transcription elongation, particularly past template-encoded arresting sites. The SIII complex, composed of elongins A/A2, B, and C, plays a crucial role in this process. By mitigating transient pausing of RNA polymerase II at various points within transcription units, the SIII complex facilitates efficient elongation. Within this complex, elongin A serves as the transcriptionally active component, while elongins B and C provide regulatory functions. Notably, elongin A2, specifically expressed in the testis, exhibits the ability to form stable complexes with elongins B and C. The tumor suppressor protein VHL (von Hippel-Lindau) interacts with elongins B and C, consequently inhibiting transcription elongation.
Description
Recombinantly produced in E.Coli, TCEB1 Human Recombinant is a single, non-glycosylated polypeptide chain. This protein consists of 132 amino acids, encompassing residues 1-112, and exhibits a molecular mass of 14.6kDa. A 20 amino acid His-tag is fused to the N-terminus of TCEB1. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The TCEB1 protein solution is provided at a concentration of 1mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT, and 0.15M NaCl.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein, such as HSA or BSA (0.1%), is advised for long-term storage to maintain stability. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is determined by SDS-PAGE analysis and is consistently greater than 90.0%.
Synonyms
Transcription elongation factor B polypeptide 1, Elongin 15 kDa subunit, Elongin-C, EloC, RNA polymerase II transcription factor SIII subunit C, SIII p15, TCEB1, SIII.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC.

Product Science Overview

Structure and Function

The SIII complex is composed of three subunits: Elongin A/A2, Elongin B, and Elongin C. Elongin A functions as the transcriptionally active component, while Elongin B and Elongin C serve as regulatory subunits . Elongin C, specifically, is a 15 kDa protein that enhances the transcriptional activity of Elongin A by suppressing transient pausing of RNA polymerase II at various sites within transcription units .

Interaction with Other Proteins

Elongin C is known to interact with the von Hippel-Lindau (VHL) tumor suppressor protein. This interaction is crucial as it inhibits transcription elongation, thereby playing a role in tumor suppression . Additionally, in embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes, generating genomic regions that display both active and repressive chromatin properties, which is essential for maintaining pluripotency .

Clinical Significance

Mutations in the TCEB1 gene have been associated with certain types of renal cell carcinoma, specifically ELOC-mutated renal cell carcinoma . This type of carcinoma is characterized by clear cells with abundant cytoplasm and basal nuclei, and it has a generally good prognosis based on limited data .

Recombinant Production

Human recombinant TCEB1 is produced using recombinant DNA technology, which involves inserting the TCEB1 gene into an expression vector, transforming it into a suitable host cell (such as E. coli or yeast), and inducing the expression of the protein. The recombinant protein is then purified using various chromatographic techniques to ensure its functionality and purity.

Applications

Recombinant TCEB1 is widely used in research to study the mechanisms of transcription elongation and its regulation. It is also used in studies related to cancer biology, particularly in understanding the role of the VHL protein and its interactions with elongin C.

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Source
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