TBCB Human

Tubulin Folding Cofactor B Human Recombinant
Cat. No.
BT20076
Source
E.coli.
Synonyms
Tubulin folding cofactor B, Cytoskeleton-associated protein CKAPI, cytoskeleton associated protein 1, CKAP1, CG22, Tubulin-specific chaperone B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TBCB Human Recombinant produced in E. coli is a single polypeptide chain containing 268 amino acids (1-244) and having a molecular mass of 29.9 kDa.
TBCB is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TBCB, a member of the Microtubules family, plays a crucial role in the biosynthesis of functional microtubules. This process involves several chaperones known as Tubulin folding cofactors A (TBCA), B (TBCB), C (TBCC), D (TBCD), and E (TBCE), which act on folding intermediates downstream of the cytosolic chaperone TCP. TBCB, a 244 amino acid cytoplasmic protein, contains a CAP-Gly domain and is ubiquitously expressed. It participates in regulating tubulin heterodimer dissociation and functions as a negative regulator of axonal growth.
Description
Recombinant TBCB Human, produced in E. coli, is a single polypeptide chain comprising 268 amino acids (1-244) with a molecular weight of 29.9 kDa. It includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The TBCB solution (0.5mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Tubulin folding cofactor B, Cytoskeleton-associated protein CKAPI, cytoskeleton associated protein 1, CKAP1, CG22, Tubulin-specific chaperone B.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEVTGV SAPTVTVFIS SSLNTFRSEK RYSRSLTIAE FKCKLELLVG SPASCMELEL YGVDDKFYSK LDQEDALLGS YPVDDGCRIH VIDHSGARLG EYEDVSRVEK YTISQEAYDQ RQDTVRSFLK RSKLGRYNEE ERAQQEAEAA QRLAEEKAQA SSIPVGSRCE VRAAGQSPRR GTVMYVGLTD FKPGYWIGVR YDEPLGKNDG SVNGKRYFEC QAKYGAFVKP AVVTVGDFPE EDYGLDEI

Product Science Overview

Function and Mechanism

TBCB is involved in the chaperonin-mediated protein folding pathway. It binds to alpha-tubulin folding intermediates after their interaction with the cytosolic chaperonin complex, leading to the formation of properly folded tubulin heterodimers . This process is essential for maintaining microtubule dynamics and stability.

In humans, the CCT (Chaperonin Containing TCP-1) complex facilitates the folding of quasi-native tubulin by alternately opening and closing two folding chambers . Along with other tubulin folding cofactors (TFCs), TBCB assists in the folding and dimerization of newly synthesized labile alpha- and beta-tubulin monomers .

Biological Importance

TBCB is predicted to be involved in cell differentiation and nervous system development . It may also function as a negative regulator of axonal growth, which is crucial for the proper development and functioning of the nervous system .

Associated Diseases

Mutations or dysregulation of TBCB have been associated with certain diseases, including Primary Ciliary Dyskinesia and Acrorenal Syndrome . These conditions highlight the importance of TBCB in maintaining cellular and physiological homeostasis.

Research and Applications

Research on TBCB has provided insights into its role in microtubule dynamics and its potential implications in various diseases. Understanding the function and regulation of TBCB can lead to the development of therapeutic strategies for conditions associated with microtubule dysfunction.

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THE BIOTEK
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