TBCB Antibody

Tubulin Folding Cofactor B, Mouse Anti Human
Cat. No.
BT1169
Source
Synonyms
Tubulin folding cofactor B, Cytoskeleton-associated protein CKAPI, cytoskeleton associated protein 1, CKAP1, CG22, Tubulin-specific chaperone B.
Appearance
Sterile filtered colorless solution.
Purity
Usage
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Description

Product Specs

Introduction
TBCB, a member of the Microtubules family, plays a crucial role in the biosynthesis of functional microtubules. This process involves several chaperones known as Tubulin folding cofactors A (TBCA), B (TBCB), C (TBCC), D (TBCD), and E (TBCE), which act on folding intermediates downstream of the cytosolic chaperon TCP. TBCB, a 244 amino acid cytoplasmic protein, contains one CAP-Gly domain and is ubiquitously expressed. Its functions include regulating tubulin heterodimer dissociation and acting as a negative regulator of axonal growth.
Physical Appearance
Sterile, colorless solution.
Formulation
1 mg/ml in PBS, pH 7.4, with 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer storage periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C or 1 month at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A starting dilution of 1:1500 is recommended.
Synonyms
Tubulin folding cofactor B, Cytoskeleton-associated protein CKAPI, cytoskeleton associated protein 1, CKAP1, CG22, Tubulin-specific chaperone B.
Purification Method
TBCB antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1F6AT.
Immunogen
Anti-human TBCB mAb, clone PAT1F6AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TBCB protein 1-244 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Introduction

Tubulin Folding Cofactor B (TBCB) is a protein that plays a crucial role in the proper folding and assembly of tubulin, which is a key component of the microtubule cytoskeleton. Microtubules are essential for various cellular processes, including cell division, intracellular transport, and maintenance of cell shape.

Gene and Protein Information

The TBCB gene, also known as CKAP1 (Cytoskeleton-Associated Protein 1), encodes the TBCB protein. This gene is located on chromosome 19 in humans . The TBCB protein is involved in the folding of alpha-tubulin intermediates after their interaction with cytosolic chaperonin, leading to the formation of properly folded tubulin heterodimers .

Function and Mechanism

TBCB binds to alpha-tubulin folding intermediates and is involved in the regulation of tubulin heterodimer dissociation . It may also function as a negative regulator of axonal growth . The protein is part of a group of cofactors (TBCA-TBCE) that play a role in microtubule biosynthesis . Specifically, TBCB enhances the folding of alpha-tubulin and contributes to the polymerization of microtubules .

Clinical Significance

Mutations or dysregulation of the TBCB gene have been associated with various diseases, including Primary Ciliary Dyskinesia and Acrorenal Syndrome . These conditions highlight the importance of TBCB in maintaining proper cellular function and structure.

Research and Applications

The mouse anti-human TBCB antibody is commonly used in research to study the expression and function of TBCB in various biological contexts. This antibody is suitable for applications such as Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), and Immunocytochemistry (ICC) . It reacts with both mouse and human samples, making it a versatile tool for studying TBCB in different model systems .

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Source
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