TARDBP (1-414) Human, His

TAR DNA Binding Protein (1-414 a.a.) Human Recombinant, His Tag
Cat. No.
BT10630
Source
E.coli.
Synonyms
ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TARDBP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 450 amino acids (1-414 a.a) and having a total molecular mass of 48.8 kDa. TARDBP is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TAR DNA Binding Protein (TARDBP) is initially known for its role as a transcriptional repressor, binding to chromosomally integrated TAR DNA and inhibiting HIV-1 transcription. Additionally, TARDBP has been found in individuals diagnosed with chronic traumatic encephalopathy (CTE), a condition often resembling ALS and linked to athletes who have suffered multiple concussions and other head injuries. TARDBP exhibits the ability to bind both DNA and RNA, playing multiple roles in transcriptional repression, pre-mRNA splicing, and translational regulation.
Description
Recombinant human TARDBP, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses 450 amino acids, including amino acids 1-414 of the TARDBP sequence, and has a molecular weight of 48.8 kDa. The protein includes a 36 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The TARDBP protein solution is provided at a concentration of 1 mg/ml. The solution is buffered with 20 mM Tris-HCl at a pH of 8.0 and also contains 10% glycerol and 0.4 M urea.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
ALS10, TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.
Source
E.coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM.

Product Science Overview

Introduction

TAR DNA Binding Protein (TARDBP), also known as TDP-43, is a protein encoded by the TARDBP gene in humans. This protein is involved in various cellular processes, including transcriptional repression, pre-mRNA splicing, and translational regulation. The recombinant form of TARDBP, specifically the 1-414 amino acid sequence with a His tag, is commonly used in research to study its functions and interactions.

Structure and Production

The recombinant human TARDBP (1-414 a.a.) is produced in E. coli as a single, non-glycosylated polypeptide chain containing 450 amino acids, including a 36 amino acid His-tag at the N-terminus . The total molecular mass of this recombinant protein is approximately 48.8 kDa . The His-tag facilitates purification through affinity chromatography, making it easier to isolate the protein for experimental purposes.

Functions

TARDBP has been shown to bind both DNA and RNA, playing multiple roles in cellular processes . Some of its key functions include:

  • Transcriptional Repression: TARDBP was initially recognized for its role in repressing HIV-1 transcription by binding to chromosomally integrated TAR DNA .
  • RNA Processing: It regulates the splicing of pre-mRNA and is involved in the stability and translation of mRNA .
  • Mitochondrial Homeostasis: TARDBP plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts .
Clinical Relevance

TARDBP is associated with several neurodegenerative diseases. Mutations in the TARDBP gene have been linked to Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD) . Additionally, TARDBP has been detected in individuals diagnosed with chronic traumatic encephalopathy (CTE), a condition often associated with athletes who have experienced multiple concussions .

Research Applications

The recombinant form of TARDBP (1-414 a.a.) with a His tag is widely used in research to study its biochemical properties and interactions. It is particularly useful for investigating the molecular mechanisms underlying its role in neurodegenerative diseases and other cellular processes .

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THE BIOTEK
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