Introduction
TAR DNA Binding Protein (TARDBP) was initially identified for its ability to bind to TAR DNA and repress HIV-1 transcription. TARDBP is also found in individuals with chronic traumatic encephalopathy, a condition that shares similarities with ALS and is linked to repeated head injuries in athletes. TARDBP can bind to both DNA and RNA and plays multiple roles in transcriptional repression, pre-mRNA splicing, and translational regulation.
Description
Recombinant human TARDBP, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 414 amino acids (1-414 a.a) with a molecular weight of 44.7 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The TARDBP protein solution is provided at a concentration of 0.25 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 M NaCl, 10% glycerol, and 1 mM EDTA.
Stability
The TARDBP protein should be stored at 4°C for up to 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein, such as HSA or BSA (0.1%), is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of TARDBP is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
ALS10,
TDP43, TAR DNA-binding protein 43, TDP-43, TARDBP.
Amino Acid Sequence
MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC MRGVRLVEGI LHAPDAGWGN LVYVVNYPKD NKRKMDETDA SSAVKVKRAV QKTSDLIVLG LPWKTTEQDL KEYFSTFGEV LMVQVKKDLK TGHSKGFGFV RFTEYETQVK VMSQRHMIDG RWCDCKLPNS KQSQDEPLRS RKVFVGRCTE DMTEDELREF FSQYGDVMDV FIPKPFRAFA FVTFADDQIA QSLCGEDLII KGISVHISNA EPKHNSNRQL ERSGRFGGNP GGFGNQGGFG NSRGGGAGLG NNQGSNMGGG MNFGAFSINP AMMAAAQAAL QSSWGMMGML ASQQNQSGPS GNNQNQGNMQ REPNQAFGSG NNSYSGSNSG AAIGWGSASN AGSGSGFNGG FGSSMDSKSS GWGM.
