TACI Human, Sf9
Tumor Necrosis Factor Receptor 13B Human Recombinant, Sf9
Cat. No.
BT24574
Source
Sf9,
Insect cells.
Synonyms
Tumor
Necrosis Factor Receptor Superfamily Member 13B, Tumor Necrosis Factor Receptor
Superfamily, Member 13B, Transmembrane Activator And CAML Interactor ,TACI ,
Tumor Necrosis Factor Receptor 13B, CD267 Antigen, TNFRSF14B, CD267, CVID2, IGAD2,
CVID, RYZN.
Appearance
Sterile
filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
| TACI produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 407 amino acids (1-165a.a.) and having a molecular mass of 45.8kDa (Molecular size on SDS-PAGE will appear at approximately 25-50kDa). TACI is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques. |
Product Specs
Introduction
TACI, or TNFRSF13B, is a transmembrane receptor protein primarily found on the surface of B cells, key components of the immune system. Initially discovered for its interaction with CAML (calcium-modulator and cyclophilin ligand), TACI is now recognized for its crucial role in humoral immunity through interactions with two TNF family members. It regulates T cell-independent B cell antibody responses, isotype switching, and maintains B cell homeostasis.
Description
TACI, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain with 407 amino acids (1-165a.a.) and a molecular mass of 45.8 kDa. Note: On SDS-PAGE, the molecular size appears between 25-50 kDa. The protein is expressed with a 242 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A colorless solution that has been sterilized by filtration.
Formulation
The TACI protein solution is provided at a concentration of 0.25 mg/ml and contains Phosphate Buffered Saline (pH 7.4), 30% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), keep the vial at 4°C. For longer storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of TACI is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Tumor
Necrosis Factor Receptor Superfamily Member 13B, Tumor Necrosis Factor Receptor
Superfamily, Member 13B, Transmembrane Activator And CAML Interactor ,TACI ,
Tumor Necrosis Factor Receptor 13B, CD267 Antigen, TNFRSF14B, CD267, CVID2, IGAD2,
CVID, RYZN.
Source
Sf9,
Insect cells.
Amino Acid Sequence
ADLMSGLGRS RRGGRSRVDQ
EERFPQGLWT GVAMRSCPEE QYWDPLLGTC MSCKTICNHQ SQRTCAAFCR SLSCRKEQGK FYDHLLRDCI SCASICGQHP
KQCAYFCENK LRSPVNLPPE LRRQRSGEVE NNSDNSGRYQ GLEHRGSEAS PALPGLKLSA DQVALVYSLE
PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN
AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD
ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS
VMHEALHNHY TQKSLSLSPG KHHHHHH.
Product Science Overview
Structure and Expression
TNFRSF13B is a transmembrane protein predominantly found on the surface of B cells . The human recombinant version of this protein, expressed in Sf9 insect cells, is a soluble 159 amino acid polypeptide with a molecular weight of approximately 17.8 kDa . The protein comprises the TNFR homologous, cysteine-rich extracellular domain of the TACI protein .
Function
TACI interacts with three ligands: APRIL (A Proliferation-Inducing Ligand), BAFF (B-cell Activating Factor), and CAML (Calcium-Modulator and Cyclophilin Ligand) . These interactions are essential for various immune responses, including:
- B cell proliferation and survival: TACI signaling promotes the survival and proliferation of B cells, which are vital for the adaptive immune response .
- Isotype switching: TACI plays a role in the process of isotype switching, where B cells change the class of antibody they produce without altering the antigen specificity .
- Humoral immunity: TACI is involved in the regulation of humoral immune responses, which are mediated by antibodies produced by B cells .
Clinical Significance
Mutations or defects in the TNFRSF13B gene can lead to immune system disorders. For instance, TACI mutations have been associated with Common Variable Immunodeficiency (CVID) and Selective IgA Deficiency (IGAD) . These conditions result in impaired antibody production and increased susceptibility to infections.
Research and Applications
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