TACI Human, His

Tumor Necrosis Factor Receptor 13B Human Recombinant, His Tag
Cat. No.
BT24706
Source
E.coli.
Synonyms
CD267, CVID, CVID2, TACI, TNFRSF14B, Tumor necrosis factor receptor superfamily, member 13B, Tumor necrosis factor receptor superfamily, member 13B, isoform CRA_a, TNFRSF13B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TACI Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 188 amino acids (1- 165a.a.) and having a molecular mass of 20.9kDa. The TACI is purified by proprietary chromatographic techniques.

Product Specs

Introduction
TNFRSF13B (TACI) is a transmembrane receptor protein primarily located on the surface of B cells, which are crucial components of the immune system. TACI was initially identified due to its interaction with calcium-modulator and cyclophilin ligand (CAML). Subsequent research revealed its critical role in humoral immunity through interactions with two members of the TNF family. TACI also regulates T cell-independent B cell antibody responses, isotype switching, and maintains B cell homeostasis.
Description
Recombinant human TACI protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 188 amino acids (including a 23 amino acid His tag at the N-terminus), encompassing amino acids 1-165 of the native sequence. The protein has a molecular weight of 20.9 kDa and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The TACI solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the TACI protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
CD267, CVID, CVID2, TACI, TNFRSF14B, Tumor necrosis factor receptor superfamily, member 13B, Tumor necrosis factor receptor superfamily, member 13B, isoform CRA_a, TNFRSF13B.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSGLGRS RRGGRSRVDQ EERFPQGLWT GVAMRSCPEE QYWDPLLGTC MSCKTICNHQ SQRTCAAFCR SLSCRKEQGK FYDHLLRDCI SCASICGQHP KQCAYFCENK LRSPVNLPPE LRRQRSGEVE NNSDNSGRYQ GLEHRGSEAS PALPGLKLSA DQVALVYS.

Product Science Overview

Structure and Function

TNFRSF13B is a transmembrane protein that interacts with calcium-modulator and cyclophilin ligand (CAML). It plays a significant role in the activation of transcription factors such as NFAT, AP1, and NF-kappa-B. These transcription factors are essential for the regulation of humoral immunity, which involves the production of antibodies by B cells .

The receptor binds to two ligands with high affinity: TNFSF13/APRIL and TNFSF13B/BAFF/BLYS. These interactions are crucial for the stimulation of B- and T-cell functions, as well as the regulation of humoral immunity .

Recombinant Protein and His Tag

The recombinant form of TNFRSF13B, tagged with a polyhistidine (His) tag, is produced using recombinant DNA technology. The His tag is a sequence of histidine residues added to the protein to facilitate its purification and detection. This tag allows for the efficient isolation of the protein using metal affinity chromatography, which is a common technique in protein purification .

Clinical Significance

Mutations in the TNFRSF13B gene have been associated with various immunodeficiency disorders, including Common Variable Immunodeficiency (CVID) and Selective IgA Deficiency (IGAD). These conditions are characterized by a reduced ability to produce antibodies, leading to increased susceptibility to infections .

The receptor’s role in immune regulation makes it a potential target for therapeutic interventions in autoimmune diseases and other conditions involving dysregulated immune responses .

Research and Applications

Research on TNFRSF13B has provided valuable insights into its role in immune regulation and its potential as a therapeutic target. The recombinant form of the protein, with the His tag, is widely used in laboratory studies to investigate its function and interactions with other molecules .

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