S.typhi Paired Antibody
Sterile filtered clear colorless solution.
Greater than 90%.
Description
Typhi antibodies, capture and conjugating, target S. Typhi Outer Membrane Protein and S.Typhi specific polysaccharide respectively.
This Antibody is used both as conjugate and capture.
Product Specs
This product features a pair of antibodies designed for the specific detection of Salmonella Typhi. The capture antibody binds to the Outer Membrane Protein of S. Typhi, while the conjugating antibody targets a polysaccharide unique to this bacterium. These antibodies are crucial components in various diagnostic assays.
This product serves a dual purpose, functioning as both a capture and conjugating antibody.
The purity level of this antibody exceeds 90%, indicating a high level of quality and reliability.
The antibody solution is sterile, ensuring its suitability for use in various applications. It is clear and colorless in appearance.
This product consists of S. Typhi capture and conjugate antibodies at a concentration of 2.7mg/1ml. It is formulated in a solution of 0.01M phosphate buffered saline with a pH of 7.2 and contains 0.1% sodium azide as a preservative.
To maintain optimal performance, the S. Typhi Paired antibody should be stored at a refrigerated temperature between 2-8 degrees Celsius.
Purified monoclonal IgG by protein A chromatography.
Product Science Overview
Salmonella Typhi is a Gram-negative bacterium responsible for causing typhoid fever, a severe and potentially life-threatening illness. Typhoid fever affects millions of people worldwide, particularly in regions with poor sanitation and limited access to clean water . The development of effective diagnostic tools and treatments is crucial in managing and controlling the spread of this disease.
Monoclonal antibodies (mAbs) are laboratory-produced molecules engineered to serve as substitute antibodies that can restore, enhance, or mimic the immune system’s attack on cells . These antibodies are designed to bind to specific antigens, in this case, the antigens present on the surface of Salmonella Typhi bacteria . Monoclonal antibodies are highly specific, targeting a single epitope on the antigen, which makes them valuable tools in both diagnostic and therapeutic applications .
The Mouse Anti-Salmonella Typhi Paired Antibody is a monoclonal antibody specifically developed to target Salmonella Typhi. This antibody is used in various laboratory research applications, including the detection and quantification of Salmonella Typhi in biological samples . The paired antibody system typically involves two antibodies: a capture antibody and a detection (or conjugate) antibody. The capture antibody binds to a specific antigen on the Salmonella Typhi bacterium, while the detection antibody binds to another epitope on the same antigen, allowing for precise identification and measurement .
The production of monoclonal antibodies involves the fusion of myeloma cells with spleen cells from a mouse immunized with the target antigen. This fusion creates hybridoma cells that can produce large quantities of the desired antibody . The antibodies are then purified using techniques such as protein A chromatography to ensure high purity and specificity .
- Diagnostic Tools: The Mouse Anti-Salmonella Typhi Paired Antibody is used in various diagnostic assays, including enzyme-linked immunosorbent assays (ELISAs) and immunofluorescence assays, to detect the presence of Salmonella Typhi in clinical samples .
- Research: These antibodies are valuable in research settings for studying the pathogenesis of Salmonella Typhi and the host immune response to infection .
- Vaccine Development: Monoclonal antibodies can be used to evaluate the efficacy of potential vaccines by measuring the immune response generated against Salmonella Typhi .
