S.Typhi HylE
Description
Product Specs
Product Science Overview
Salmonella enterica serovar Typhi (S. Typhi) is the causative agent of typhoid fever, a systemic infection that affects millions of people worldwide annually. One of the key virulence factors of S. Typhi is Hemolysin E (HlyE), a pore-forming toxin that plays a significant role in the pathogenesis of the disease .
Hemolysin E is an immunogenic protein that contributes to the cytotoxicity and invasion of epithelial cells. It is involved in the bacterial growth within human macrophages and affects the overall virulence of S. Typhi . The protein forms pores in the host cell membranes, leading to cell lysis and aiding in the spread of the bacteria.
Recombinant HlyE refers to the HlyE protein that has been expressed and purified using recombinant DNA technology. This involves cloning the hlyE gene from S. Typhi into a suitable expression vector, such as pET28a, and transforming it into a host organism like Escherichia coli (E. coli) for protein production . The recombinant HlyE protein can then be purified and used for various research and diagnostic purposes.
- Diagnostic Tool: Recombinant HlyE has been evaluated as a potential biomarker for the detection of typhoid fever. Studies have shown that antibodies against HlyE can be detected in the sera of patients with acute typhoid fever, making it a useful tool for serodiagnosis .
- Vaccine Development: Due to its immunogenic properties, recombinant HlyE is being explored as a potential candidate for vaccine development against typhoid fever. The identification of key immunogenic regions of HlyE can aid in the design of effective vaccines .
- Therapeutic Target: Mapping of B-cell epitopes on HlyE has identified regions that can be targeted by monoclonal antibodies. These antibodies can potentially neutralize the toxin and provide therapeutic benefits for typhoid fever patients .
