STX1A (1-265) Human

Syntaxin-1A (1-265 a.a) Human Recombinant
Cat. No.
BT8534
Source
Escherichia Coli.
Synonyms
Syntaxin 1A, Neuron-Specific Antigen HPC-1, Syntaxin 1A (Brain), STX1, Syntaxin-1A, HPC-1, P35-1, SYN1A.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

STX1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-265 a.a) and having a molecular mass of 30.7kDa. 
STX1A is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Syntaxin is a Q-SNARE protein found in the membrane that plays a crucial role in exocytosis. It comprises three main domains: an N-terminal regulatory domain (Habc), a SNARE domain (H3), and a single C-terminal transmembrane domain. The SNARE domain interacts with synaptobrevin and SNAP-25 to form the core SNARE complex, which is essential for vesicle fusion. During calcium-regulated exocytosis, synaptic vesicles release neurotransmitters. Syntaxins contribute to this process by ensuring the localization of synaptic vesicles and calcium channels to the presynaptic active zone, thereby enabling specific neurotransmitter release. Furthermore, syntaxins act as a substrate for botulinum neurotoxin type C, a metalloprotease known to inhibit exocytosis. This neurotoxin exhibits a strong affinity for a molecular complex that includes the alpha-latrotoxin receptor, which is involved in exocytosis.
Description
Recombinant Human STX1A, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 265 amino acids (1-265 a.a.). It has a molecular weight of 30.7 kDa. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The STX1A protein solution (1 mg/ml) is supplied in a buffer containing phosphate buffered saline (pH 7.4), 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the protein is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Syntaxin 1A, Neuron-Specific Antigen HPC-1, Syntaxin 1A (Brain), STX1, Syntaxin-1A, HPC-1, P35-1, SYN1A.
Source
Escherichia Coli.
Amino Acid Sequence
MKDRTQELRT AKDSDDDDDV AVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD TKKAVKYQSK ARRKK.

Product Science Overview

Structure and Expression

The human recombinant Syntaxin-1A (1-265 a.a) is a full-length protein comprising 265 amino acids. It is expressed in Escherichia coli and has a purity greater than 95%, making it suitable for various biochemical applications such as SDS-PAGE . The protein is also known by several alternative names, including Neuron-specific antigen HPC-1, STX1A, and STX1 .

Function and Mechanism

Syntaxin-1A is primarily found in the brain and is essential for synaptic vesicle exocytosis. It interacts with other SNARE proteins, such as SNAP-25 and VAMP (vesicle-associated membrane protein), to form a SNARE complex. This complex brings the vesicle and plasma membranes close together, facilitating their fusion and the subsequent release of neurotransmitters .

The protein’s structure includes a transmembrane domain that anchors it to the plasma membrane and a cytoplasmic domain that interacts with other SNARE proteins. The interaction between Syntaxin-1A and its partners is highly regulated and crucial for the precise timing of neurotransmitter release, which is essential for proper neuronal communication .

Applications and Research

Recombinant Syntaxin-1A (1-265 a.a) is widely used in research to study the mechanisms of synaptic transmission and the role of SNARE proteins in vesicle fusion. It is also used in various assays to investigate the interactions between SNARE proteins and other regulatory factors involved in neurotransmitter release .

Understanding the function and regulation of Syntaxin-1A is important for elucidating the molecular basis of synaptic transmission and for developing potential therapeutic strategies for neurological disorders that involve synaptic dysfunction.

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Syntaxin-1A Human Recombinant
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Source
Escherichia Coli.
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