STAT1 Human

Signal Transducer and Activator of Transcription 1 Human Recombinant
Cat. No.
BT14807
Source
Escherichia Coli.
Synonyms
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1, ISGF-3, STAT91, DKFZp686B04100.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

STAT1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 732 amino acids (1-712 a.a.) and having a molecular mass of 85.2 kDa. The STAT1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
STAT1, a transcription factor belonging to the Signal Transducers and Activators of Transcription family, plays a crucial role in gene upregulation in response to type I or type II interferons. When stimulated by IFN-γ, STAT1 forms homodimers or heterodimers with STAT3, binding to the GAS (Interferon-Gamma Activated Sequence) promoter element. Conversely, stimulation by either IFN-α or IFN-β leads to STAT1 forming a heterodimer with STAT2, which binds to the ISRE (Interferon Stimulated Response Element) promoter element. Both scenarios result in promoter element binding, ultimately increasing the expression of ISGs (Interferon Stimulated Genes).
Description
Recombinant Human STAT1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 732 amino acids (specifically, amino acids 1-712). It possesses a molecular weight of 85.2 kDa. A 20 amino acid His-Tag is fused to the N-terminus of STAT1. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The STAT1 protein solution has a concentration of 0.5mg/ml and contains 20mM Tris-HCl buffer (pH 8), 0.1M NaCl, 1mM DTT, and 10% glycerol.
Stability
For optimal storage, keep the vial refrigerated at 4°C if it will be completely used within 2-4 weeks. For extended storage, freeze the solution at -20°C. To maintain product integrity, avoid repeated cycles of freezing and thawing.
Purity
SDS-PAGE analysis indicates a purity greater than 90%.
Synonyms
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1, ISGF-3, STAT91, DKFZp686B04100.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV SFATIRFHDL LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ MSMIIYSCLK EERKILENAQ RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR EHETNGVAKS DQKQEQLLLK KMYLMLDNKR KEVVHKIIEL LNVTELTQNA LINDELVEWK RRQQSACIGG PPNACLDQLQ NWFTIVAESL QQVRQQLKKL EELEQKYTYE HDPITKNKQV LWDRTFSLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKLRLLVK LQELNYNLKV KVLFDKDVNE RNTVKGFRKF NILGTHTKVM NMEESTNGSL AAEFRHLQLK EQKNAGTRTN EGPLIVTEEL HSLSFETQLC QPGLVIDLET TSLPVVVISN VSQLPSGWAS ILWYNMLVAE PRNLSFFLTP PCARWAQLSE VLSWQFSSVT KRGLNVDQLN MLGEKLLGPN ASPDGLIPWT RFCKENINDK NFPFWLWIES ILELIKKHLL PLWNDGCIMG FISKERERAL LKDQQPGTFL LRFSESSREG AITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN YKVMAAENIP ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDGPKGTG YIKTELISVS EV.

Product Science Overview

Introduction

Signal Transducer and Activator of Transcription 1 (STAT1) is a crucial protein in the STAT family of transcription factors. It plays a significant role in mediating cellular responses to various cytokines and growth factors, including interferon-alpha (IFN-α), interferon-gamma (IFN-γ), epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and interleukin-6 (IL-6) .

Structure and Function

STAT1 is a cytosolic protein composed of approximately 750 to 800 amino acids. It has a common structure that includes an N-terminal oligomerization domain, a DNA-binding domain, and a C-terminal SRC homology-2 (SH2) domain. The SH2 domain is involved in the association between STATs and receptors .

Upon activation by cytokines, STAT1 undergoes phosphorylation, leading to the formation of homo- or heterodimers. These dimers translocate to the cell nucleus, where they act as transcription activators, regulating the expression of various genes involved in cell growth, apoptosis, immune responses, and lipid metabolism .

Activation and Signaling Pathways

STAT1 can be activated by several ligands, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. The activation process involves the phosphorylation of STAT1, which then forms homodimers or heterodimers with other STAT family members. These dimers translocate to the nucleus and bind to specific DNA elements, such as the gamma-activating sequence (GAS) and the interferon-stimulated response element (ISRE), to regulate gene transcription .

Biological Significance

STAT1 plays a vital role in immune responses to viral, fungal, and mycobacterial pathogens. It mediates the expression of genes that are crucial for cell viability in response to different cell stimuli and pathogens. Mutations in the STAT1 gene are associated with various immunodeficiency disorders, including Immunodeficiency 31A, 31B, and 31C .

Recombinant STAT1

Human recombinant STAT1 is a form of the protein that is produced using recombinant DNA technology. This technology allows for the production of large quantities of STAT1 protein for research and therapeutic purposes. Recombinant STAT1 is used in various studies to understand its role in cellular signaling and to develop potential treatments for diseases associated with STAT1 dysfunction .

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