STAT1 Antibody
Description
Product Specs
Product Science Overview
Signal Transducer and Activator of Transcription 1 (STAT1) is a transcription factor encoded by the STAT1 gene. It is a member of the STAT protein family, which plays a crucial role in mediating cellular responses to cytokines and growth factors . STAT1 is involved in various biological processes, including immune responses, cell growth, differentiation, and apoptosis.
STAT1 exists in two main isoforms due to alternative splicing: STAT1α (91 kDa) and STAT1β (84 kDa). STAT1α is the full-length version and is primarily responsible for most of the known functions of STAT1. STAT1β lacks a portion of the C-terminus and has been reported to either negatively regulate STAT1 activation or mediate specific anti-tumor and anti-infection activities .
STAT1 is activated by phosphorylation through receptor-associated kinases in response to various ligands, including interferons (IFN-α, IFN-γ), epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and interleukins (IL-6, IL-27) . Upon activation, STAT1 forms homo- or heterodimers, translocates to the nucleus, and binds to specific DNA sequences to regulate gene expression .
STAT1 plays a key role in upregulating genes involved in cell survival, pathogen response, and immune regulation. It is particularly important in the immune response to viral, fungal, and mycobacterial pathogens .
Mouse anti-human STAT1 antibodies are monoclonal or polyclonal antibodies developed in mice to specifically target and bind to the human STAT1 protein. These antibodies are widely used in various scientific applications, including Western blotting, immunocytochemistry, immunohistochemistry, flow cytometry, and immunoprecipitation .
Mouse anti-human STAT1 antibodies are designed to detect endogenous levels of total STAT1 protein, independent of its phosphorylation status. Some antibodies, such as the Stat1 (9H2) Mouse mAb, prefer the non-phosphorylated form of STAT1 and can detect both STAT1α and STAT1β isoforms . These antibodies do not significantly cross-react with other STAT proteins, ensuring specificity in experimental applications .
