SSR4 Human

Signal Sequence Receptor, Delta Human Recombinant
Cat. No.
BT26969
Source
Escherichia Coli.
Synonyms
TRAPD, Translocon-associated protein subunit delta, TRAP-delta, Signal sequence receptor subunit delta SSR-delta.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

SSR4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 144 amino acids (24-144 a.a.) and having a molecular mass of 16.1kDa.
SSR4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
SSR4, a member of the TRAP-delta family, serves as the delta subunit within the translocon-associated protein complex. This complex plays a crucial role in protein translocation across the endoplasmic reticulum membrane. Located in the Xq28 region, the SSR4 gene exhibits a compact head-to-head arrangement with the gene encoding isocitrate dehydrogenase 3 (NAD+) gamma. Notably, both genes share a common CpG-embedded bidirectional promoter.
Description
Recombinant human SSR4, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 144 amino acids (specifically, residues 24-144). With a molecular weight of 16.1 kDa, this protein features a 23-amino acid His-tag fused to its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, sterile, and filtered solution.
Formulation
The SSR4 protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the SSR4 protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
TRAPD, Translocon-associated protein subunit delta, TRAP-delta, Signal sequence receptor subunit delta SSR-delta.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEACLEPQ ITPSYYTTSD AVISTETVFI VEISLTCKNR VQNMALYADV GGKQFPVTRG QDVGRYQVSW SLDHKSAHAG TYEVRFFDEE SYSLLRKAQR NNEDISIIPP LFTVSVDHRG TWNG.

Product Science Overview

Introduction

Signal Sequence Receptor, Delta (SSR4), also known as the delta subunit of the translocon-associated protein complex, plays a crucial role in the translocation of proteins across the endoplasmic reticulum (ER) membrane. This protein is essential for the proper functioning of cellular processes and is involved in various biological pathways.

Structure and Function

The SSR4 gene encodes the delta subunit of the translocon-associated protein complex. This complex is responsible for the translocation of newly synthesized proteins across the ER membrane. The delta subunit, along with other subunits, forms a channel through which proteins can pass into the ER lumen. The SSR4 protein is located in the Xq28 region of the human genome and is arranged in a compact head-to-head manner with the isocitrate dehydrogenase 3 (NAD+) gamma gene .

Recombinant Human SSR4

Recombinant human SSR4 is produced using recombinant DNA technology, which involves inserting the SSR4 gene into a suitable expression system, such as E. coli or HEK293 cells. The recombinant protein is then purified and used for various research applications. The recombinant human SSR4 protein typically includes a His-tag or other tags to facilitate purification and detection .

Applications

Recombinant human SSR4 is used in various research applications, including:

  • Protein translocation studies: Understanding the mechanism of protein translocation across the ER membrane.
  • Protein-protein interactions: Investigating interactions between SSR4 and other proteins involved in the translocation process.
  • Disease research: Studying the role of SSR4 in diseases related to protein translocation defects.
Clinical Significance

Mutations in the SSR4 gene can lead to various diseases, including congenital disorders of glycosylation (CDG). CDG is a group of inherited metabolic disorders that affect the glycosylation of proteins and lipids. Patients with SSR4 mutations may exhibit symptoms such as developmental delay, intellectual disability, and other systemic manifestations .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

SSR4 Antibody
Signal Sequence Receptor Delta, Mouse Anti Human
Cat. No.
BT602
Source
SSR1 Human
Signal Sequence Receptor, Alpha Human Recombinant
Cat. No.
BT26789
Source
Escherichia Coli.
SSR2 Human
Signal Sequence Receptor, Beta Human Recombinant
Cat. No.
BT26889
Source
E.coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.