SSR1 Human

Signal Sequence Receptor, Alpha Human Recombinant
Cat. No.
BT26789
Source
Escherichia Coli.
Synonyms
Signal Sequence Receptor Alpha, Translocon-Associated Protein Alpha, SSR Alpha Subunit, TRAP-alpha, TRAPA.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SSR1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain topological domain containing 209 amino acids (22-207 a.a) and having a molecular mass of 23.1kDa. SSR1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The signal sequence receptor (SSR) is a glycosylated receptor found on the endoplasmic reticulum (ER) membrane. It plays a crucial role in protein translocation across the ER membrane. SSR consists of two subunits: a 34-kD glycoprotein encoded by the SSR gene and a 22-kD glycoprotein. The SSR gene produces multiple mRNA transcripts due to complex alternative polyadenylation, utilizing an array of mostly non-canonical polyA signal sequences.
Description
Recombinant human SSR1 protein was produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 209 amino acids (amino acids 22-207) with a molecular mass of 23.1 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The SSR1 protein solution is supplied at a concentration of 0.25 mg/ml. It is formulated in 20 mM Tris-HCl buffer with a pH of 8.0, 0.1 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For optimal storage, the protein solution should be kept at 4°C if it will be used within 2-4 weeks. For long-term storage, it is recommended to store the solution frozen at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
Purity of the SSR1 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Signal Sequence Receptor Alpha, Translocon-Associated Protein Alpha, SSR Alpha Subunit, TRAP-alpha, TRAPA.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSRGGPRGL LAVAQDLTED EETVEDSIIE DEDDEAEVEE DEPTDLVEDK EEEDVSGEPE ASPSADTTIL FVKGEDFPAN NIVKFLVGFT NKGTEDFIVE SLDASFRYPQ DYQFYIQNFT ALPLNTVVPP QRQATFEYSF IPAEPMGGRP FGLVINLNYK DLNGNVFQDA VFNQTVTVIE REDGLDGET

Product Science Overview

Introduction

The Signal Sequence Receptor, Alpha (SSRA), also known as SSR1, is a crucial component of the signal sequence receptor complex located in the endoplasmic reticulum (ER) membrane. This receptor plays a significant role in the translocation of proteins across the ER membrane, a process essential for proper protein folding and function.

Structure and Composition

The SSR complex is composed of two subunits: a 34-kDa glycoprotein encoded by the SSR1 gene and a 22-kDa glycoprotein. The SSR1 gene is known for its complex alternative polyadenylation, resulting in multiple mRNA species and various isoforms . The SSR1 gene is located on chromosome 6 and is highly conserved across different species, indicating its fundamental role in cellular processes.

Function

The primary function of the SSRA is to facilitate the translocation of nascent polypeptides into the ER lumen. This process is critical for the proper folding and post-translational modifications of proteins. The SSRA binds to the signal sequence of the nascent polypeptide, guiding it to the translocon complex in the ER membrane. Once the polypeptide is translocated, the SSRA may also play a role in recycling the translocation apparatus or act as a membrane-bound chaperone to assist in protein folding .

Role in Cellular Processes

The SSRA is involved in several key cellular processes, including the Unfolded Protein Response (UPR) and cellular responses to stimuli. The UPR is a cellular stress response related to the ER, which is activated in response to the accumulation of unfolded or misfolded proteins in the ER lumen. By facilitating proper protein folding and translocation, the SSRA helps maintain ER homeostasis and prevent cellular stress .

Clinical Significance

Mutations or dysregulation of the SSR1 gene have been associated with various diseases, including Fanconi Renotubular Syndrome 5. This highlights the importance of SSRA in maintaining normal cellular function and its potential as a therapeutic target for related disorders .

Recombinant SSRA

Recombinant SSRA is produced using recombinant DNA technology, which allows for the expression of the SSRA protein in various host systems. This technology is essential for studying the protein’s structure, function, and interactions in a controlled environment. Recombinant SSRA can be used in research to understand its role in protein translocation and its potential implications in disease mechanisms.

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