SPSB2 Human

SPSB2 is characterized by the presence of a central SPRY domain (repeats in splA and RyR) and a C-terminal SOCS box . The SPRY domain is involved in protein-protein interactions, while the SOCS box is responsible for recruiting an E3 ubiquitin ligase complex. This complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins .

The gene encoding SPSB2 is located on chromosome 12p13, in a gene-rich cluster near the CD4 antigen and triosephosphate isomerase genes . This location suggests that SPSB2 may have important regulatory functions in immune responses and metabolic processes.

SPSB2 functions as a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex . This complex targets specific proteins for ubiquitination, marking them for degradation by the proteasome. One of the known targets of SPSB2 is inducible nitric oxide synthase (iNOS), which is involved in the production of nitric oxide, a signaling molecule with various physiological roles .

Recombinant Human SPRY Domain-Containing SOCS Cox Protein 2 is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 287 amino acids . The recombinant protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques . The resulting protein has a molecular mass of approximately 31.2 kDa .

Recombinant SPSB2 is primarily used for research purposes, particularly in studies related to cytokine signaling, protein ubiquitination, and immune regulation . It is also valuable in investigating the mechanisms of protein degradation and the role of SOCS proteins in various physiological and pathological processes.

The recombinant protein is typically supplied in a solution containing Tris-HCl buffer, sodium chloride, glycerol, and dithiothreitol (DTT) to maintain stability . It should be stored at 4°C if used within 2-4 weeks, or frozen at -20°C for longer periods. To prevent degradation, it is recommended to avoid multiple freeze-thaw cycles and to add a carrier protein for long-term storage .