SNX1 Human
Sorting Nexin 1 Human Recombinant
Cat. No.
BT2039
Source
Escherichia Coli.
Synonyms
HsT17379, SNX1A, Vps5.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
SNX1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 415 amino acids (146-522 a.a.) and having a molecular mass of 48 kDa. SNX1 is fused to 38 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
SNX1, a member of a large family of hydrophilic proteins, interacts with various receptor types involved in intracellular trafficking. Both SNX1 and its splice variant, SNX1A, bind to the epidermal growth factor receptor (EGFR), facilitating its transportation to the lysosome for degradation. SNX1 exhibits an association with cellular membranes and interacts with epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and receptor tyrosine kinases.
Description
Recombinant human SNX1, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 415 amino acids (146-522 a.a.). With a molecular weight of 48 kDa, it consists of SNX1 fused to a 38 a.a. His-Tag at its N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A colorless solution that has undergone sterile filtration.
Formulation
The SNX1 solution, at a concentration of 0.25 mg/ml, is formulated in a buffer containing 20mM Tris-HCl (pH 8), 0.1M NaCl, 1mM DTT, and 10% glycerol.
Stability
While SNX1 remains stable at 4°C for up to one week, storage at temperatures below -18°C is recommended. For extended storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeding 90.0% as assessed by SDS-PAGE.
Synonyms
HsT17379, SNX1A, Vps5.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGGSMTV GITDPEKIGD GMNAYVAYKV TTQTSLPLFR SKQFAVKRRF SDFLGLYEKL
SEKHSQNGFI VPPPPEKSLI GMTKVKVGKE DSSSAEFLEK RRAALERYLQ RIVNHPTMLQ DPDVREFLEK EELPRAVGTQ TLSGAGLLKM
FNKATDAVSK MTIKMNESDI WFEEKLQEVE CEEQRLRKLH AVVETLVNHR KELALNTAQF AKSLAMLGSS EDNTALSRAL SQLAEVEEKI
EQLHQEQANN DFFLLAELLS DYIRLLAIVR AAFDQRMKTW QRWQDAQATL QKKREAEARL LWANKPDKLQ QAKDEILEWE SRVTQYERDF
ERISTVVRKE VIRFEKEKSK DFKNHVIKYL ETLLYSQQQL AKYWEAFLPE AKAIS.
SEKHSQNGFI VPPPPEKSLI GMTKVKVGKE DSSSAEFLEK RRAALERYLQ RIVNHPTMLQ DPDVREFLEK EELPRAVGTQ TLSGAGLLKM
FNKATDAVSK MTIKMNESDI WFEEKLQEVE CEEQRLRKLH AVVETLVNHR KELALNTAQF AKSLAMLGSS EDNTALSRAL SQLAEVEEKI
EQLHQEQANN DFFLLAELLS DYIRLLAIVR AAFDQRMKTW QRWQDAQATL QKKREAEARL LWANKPDKLQ QAKDEILEWE SRVTQYERDF
ERISTVVRKE VIRFEKEKSK DFKNHVIKYL ETLLYSQQQL AKYWEAFLPE AKAIS.
Product Science Overview
Structure and Molecular Characteristics
- Molecular Weight: Recombinant Human Sorting Nexin 1 has a calculated molecular mass of approximately 63 kDa. However, due to abundant glycosylation, it migrates as an approximately 80 kDa protein band in SDS-PAGE under reducing conditions .
- Domains: SNX1 contains a Phox (PX) domain, which is responsible for binding to phosphoinositides, and a BAR (Bin/Amphiphysin/Rvs) domain, which is involved in membrane curvature and tubulation.
Function and Mechanism
SNX1 is primarily involved in the endosomal sorting of membrane proteins. It interacts with various receptors and other sorting nexins to form complexes that facilitate the trafficking of these proteins. Some of the key functions include:
- Receptor Recycling: SNX1 is involved in the recycling of receptors such as the epidermal growth factor receptor (EGFR) from endosomes back to the plasma membrane.
- Endosomal Sorting: It plays a role in the sorting of proteins within endosomes, directing them either towards recycling pathways or towards lysosomal degradation.
- Membrane Tubulation: The BAR domain of SNX1 helps in the formation of membrane tubules, which are essential for the transport of cargo within the cell.
Biological Significance
The proper functioning of SNX1 is critical for maintaining cellular homeostasis. Dysregulation of SNX1 and its associated pathways can lead to various diseases, including cancer and neurodegenerative disorders. Research into SNX1 and its mechanisms continues to provide insights into its role in cellular processes and its potential as a therapeutic target.
Applications
Recombinant Human Sorting Nexin 1 is used in various research applications, including:
- Protein-Protein Interaction Studies: To study the interactions between SNX1 and other proteins involved in endosomal sorting.
- Cellular Trafficking Research: To investigate the mechanisms of receptor recycling and endosomal sorting.
- Disease Models: To understand the role of SNX1 in diseases and to develop potential therapeutic strategies.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
