SNRPF Human
Description
SNRPF is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Small Nuclear Ribonucleoprotein Polypeptide F (SNRPF) is a protein encoded by the SNRPF gene in humans. This protein is a crucial component of the spliceosomal small nuclear ribonucleoproteins (snRNPs), which are essential for the splicing of pre-mRNA . The recombinant form of this protein is produced using recombinant DNA technology, which allows for the expression of the human protein in a host organism, typically bacteria or yeast.
SNRPF plays a significant role in the assembly and function of the spliceosome, a complex responsible for the removal of introns from pre-mRNA transcripts . It is a core component of the U1, U2, U4, and U5 snRNPs, which are the building blocks of the spliceosome . Additionally, SNRPF is involved in the splicing of U12-type introns in pre-mRNAs and in histone 3’-end processing as part of the U7 snRNP .
The recombinant form of SNRPF is typically produced using recombinant DNA technology. The process involves the following steps:
- Gene Cloning: The SNRPF gene is cloned into an expression vector, which is then introduced into a host organism such as Escherichia coli or Saccharomyces cerevisiae.
- Protein Expression: The host organism is cultured under conditions that induce the expression of the SNRPF protein.
- Protein Purification: The expressed protein is purified using techniques such as affinity chromatography, which exploits the specific binding properties of the protein to isolate it from other cellular components.
SNRPF is involved in several biochemical processes, primarily related to RNA splicing. It interacts with other snRNP proteins and RNA molecules to form the spliceosome complex . The protein’s interactions and functions can be studied using various biochemical and biophysical techniques, including:
- Co-immunoprecipitation: To study protein-protein interactions.
- RNA Immunoprecipitation: To investigate protein-RNA interactions.
- X-ray Crystallography and NMR Spectroscopy: To determine the three-dimensional structure of the protein and its complexes.
Mutations or dysregulation of the SNRPF gene have been associated with certain diseases, including spinal muscular atrophy . Understanding the structure and function of SNRPF can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of therapeutic interventions.
