SNRPE Human
Small Nuclear Ribonucleoprotein Polypeptide E Human Recombinant
Cat. No.
BT1576
Source
Escherichia Coli.
Synonyms
Small nuclear ribonucleoprotein E, snRNP-E, Sm protein E, Sm-E, SmE, SNRPE, B-raf.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SNRPE Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 112 amino acids (1-92 a.a.) and having a molecular mass of 12.9kDa. The SNRPE is purified by proprietary chromatographic techniques.
Product Specs
Introduction
SNRPE plays a crucial role in the assembly of UsnRNPs (U small nuclear ribonucleoproteins) within the cytoplasm. It achieves this by binding to a conserved Sm site on UsnRNA, leading to the formation of a stable snRNP core complex. As a core protein of UsnRNP, SNRPE interacts with all members of the U family of snRNAs, including U1 through U6. Additionally, SNRPE interacts with other proteins such as DDX20 and Small nuclear ribonucleoprotein polypeptide F.
Description
This product consists of the human recombinant SNRPE protein, fused with a 20 amino acid His tag at its N-terminus. It is produced in E.Coli and is a single, non-glycosylated polypeptide chain. The protein comprises 112 amino acids (specifically, amino acids 1 to 92 of the SNRPE sequence) and has a molecular weight of 12.9kDa. Purification of SNRPE is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The SNRPE solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA, and 30% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid repeated freezing and thawing of the product.
Purity
The purity of the SNRPE protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Small nuclear ribonucleoprotein E, snRNP-E, Sm protein E, Sm-E, SmE, SNRPE, B-raf.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN.
Product Science Overview
Gene and Protein Structure
Function and Mechanism
SNRPE is involved in several key processes within the cell:
- Pre-mRNA Splicing: As a core component of the spliceosomal U1, U2, U4, and U5 snRNPs, SNRPE is integral to the splicing of pre-mRNA . This process involves the removal of non-coding sequences (introns) and the joining of coding sequences (exons) to form mature mRNA.
- Histone 3’ End Processing: SNRPE is also part of the U7 snRNP, which is involved in the 3’ end processing of histone transcripts . Histones are proteins that package and order DNA into structural units called nucleosomes.
- Minor Spliceosome: SNRPE is a component of the minor spliceosome, which is responsible for the splicing of U12-type introns in pre-mRNAs .
Clinical Significance
Mutations in the SNRPE gene have been associated with several disorders:
Research and Applications
Recombinant SNRPE protein is used in various research applications, including studies on RNA splicing, autoimmune diseases, and gene expression regulation. The availability of human recombinant SNRPE allows researchers to investigate its function and interactions in a controlled environment, providing insights into its role in cellular processes and disease mechanisms.
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